WBP11 explained

Symbol:Wbp11
Wbp11
Pfam:PF09429
Interpro:IPR019007

Alternative names

Function

Studies suggest that Wbp11 plays a role in DNA/ RNA transcriptional or post-transcriptional events related to cell division.[1] Wbp11 is found in the nucleus but not the nucleoli of cells in interphase. However it is distributed throughout the cytoplasm in dividing cells.[2] Immunoelectron-microscopy experiments suggest that relocation from a peri-nuclear to a cytoplasmic distribution, coinciding with the onset of mitosis in cell division. Other studies have shown that Wbp11 is a component of the spliceosome. Also, that Wbp11 fragments block pre-mRNA splicing catalysis.[3]

Protein interactions

Wbp11 is a polypeptide known to interact with other WW domain of proteins such as the nuclear protein Npw38 via two proline-rich regions. It associates with Npw38 (hence the name NpwBP) in the nuclei and with Poly(rG) and G-rich ssDNA. The 70kDa protein has also been found to interact with SH3 (Src homology domain 3) domains. The C-terminal proline-rich sequences of SNP70/NpwBP/Wbp11, which binds to the WW domain of Npw38 also fits with both classic type I and type II SH3 binding sequences, hence the name (SNP70).

Wbp11 was found to bind strongly to the tandem SH3 domains of p47phox and to the N-terminal SH3 domain of p47phox, and more weakly to the SH3 domains from c-src and p85α. p47phox.

Furthermore, it has been shown to interact with PP1(protein phosphotase 1), hence the name SIPP1. It has an inhibitory effect to PP1, with its inhibitory potency increasing upon phosphorylation with protein kinase CK1. The binding of Wbp11 with PP1 involves a RVXF (Arg-Val-Xaa-Phe) motif, which functions as a PP1- binding sequence in most interactors of PP1.

A number of other interactions have been indicated such as:

Notes and References

  1. Komuro A, Saeki M, Kato S . Association of two nuclear proteins, Npw38 and NpwBP, via the interaction between the WW domain and a novel proline-rich motif containing glycine and arginine . The Journal of Biological Chemistry . 274 . 51 . 36513–9 . December 1999 . 10593949 . 10.1074/jbc.274.51.36513. free.
  2. Craggs G, Finan PM, Lawson D, Wingfield J, Perera T, Gadher S, Totty NF, Kellie S . A nuclear SH3 domain-binding protein that colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks . The Journal of Biological Chemistry . 276 . 32 . 30552–60 . August 2001 . 11375989 . 10.1074/jbc.M103142200 . 21245372 . free .
  3. Llorian M, Beullens M, Andrés I, Ortiz JM, Bollen M . SIPP1, a novel pre-mRNA splicing factor and interactor of protein phosphatase-1 . The Biochemical Journal . 378 . Pt 1 . 229–38 . February 2004 . 14640981 . 1223944 . 10.1042/BJ20030950 .
  4. Rolland T, Taşan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabási AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP, Vidal M . 6 . A proteome-scale map of the human interactome network . Cell . 159 . 5 . 1212–26 . November 2014 . 25416956 . 4266588 . 10.1016/j.cell.2014.10.050 .
  5. Dolan PT, Zhang C, Khadka S, Arumugaswami V, Vangeloff AD, Heaton NS, Sahasrabudhe S, Randall G, Sun R, LaCount DJ . Identification and comparative analysis of hepatitis C virus-host cell protein interactions . Molecular BioSystems . 9 . 12 . 3199–209 . December 2013 . 24136289 . 4171131 . 10.1039/c3mb70343f .
  6. Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Taşan M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barabási AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M . 6 . An inter-species protein-protein interaction network across vast evolutionary distance . Molecular Systems Biology . 12 . 4 . 865 . April 2016 . 27107014 . 4848758 . 10.15252/msb.20156484.
  7. Hegele A, Kamburov A, Grossmann A, Sourlis C, Wowro S, Weimann M, Will CL, Pena V, Lührmann R, Stelzl U . Dynamic protein-protein interaction wiring of the human spliceosome . Molecular Cell . 45 . 4 . 567–80 . February 2012 . 22365833 . 10.1016/j.molcel.2011.12.034 . free .
  8. Kofler M, Motzny K, Beyermann M, Freund C . Novel interaction partners of the CD2BP2-GYF domain . The Journal of Biological Chemistry . 280 . 39 . 33397–402 . September 2005 . 16000308 . 10.1074/jbc.M503989200 . free .
  9. Lim J, Hao T, Shaw C, Patel AJ, Szabó G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabási AL, Vidal M, Zoghbi HY . A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration . Cell . 125 . 4 . 801–14 . May 2006 . 16713569 . 10.1016/j.cell.2006.03.032 . 13709685 . free .

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