| width=4% | | width=6% | Portrait | width=23% | Governor | width=11% | From | width=11% | Until | width=15% | Monarch |
|---|
| 1 | | Blasco Núñez Vela | 15 May 1544 | 18 January 1546 |
Charles I |
| | Pedro de la Gasca,
Dean of the Audiencia | 10 April 1547 | 27 January 1550 |
| 2 | | Antonio de Mendoza,
Marquis of Mondéjar, Count of Tendilla | 23 September 1551 | 21 July 1552 |
| | Melchor Bravo de Saravia,
Dean of the Audiencia | July 1552 | June 1556 |
| 3 | | Andrés Hurtado de Mendoza,
3rd Marquis of Cañete | 29 June 1556 | 30 March 1561 |
Philip II |
| 4 | | Diego López de Zúñiga,
4th Count of Nieva | 17 April 1561 | 20 February 1564 |
| | Juan de Saavedra,
Dean of the Audiencia | 20 February 1564 | 2 September 1564 |
| | Lope García de Castro,
Dean of the Audiencia | 2 September 1564 | 26 November 1569 |
| 5 | | Francisco Álvarez de Toledo | 26 November 1569 | 23 September 1581 |
| 6 | | Martín Enríquez de Almanza | 23 September 1581 | 13 March 1583 |
| | Cristóbal Ramírez de Cartagena,
Dean of the Audiencia | 13 March 1583 | 31 March 1584 |
| 7 | | Fernando Torres de Portugal y Mesía,
1st Count of Villadompardo | 31 March 1584 | 20 November 1589 |
| 8 | | García Hurtado de Mendoza,
5th Marquis of Cañete | 8 January 1590 | 24 July 1596 |
| 9 | | Luis de Velasco,
Marquis of Salinas | 24 July 1596 | 18 January 1604 |
| 10 | | Gaspar de Zúñiga,
5th Count of Monterrey | 18 January 1604 | 16 March 1606 |
Philip III |
| | Diego Núñez de Avendaño,
Dean of the Audiencia | 16 March 1606 | 21 December 1607 |
| 11 | | Juan de Mendoza,
3rd Marquis of Montesclaros | 21 December 1607 | 18 December 1615 |
| 12 | | Francisco de Borja y Aragón,
Count of Rebolledo, Prince of Esquilache | 18 December 1615 | 31 December 1621 |
| | Juan Jiménez de Montalvo,
Dean of the Audiencia | 31 December 1621 | 25 July 1622 |
Philip IV |
| 13 | | Diego Fernández de Córdoba,
Marquis of Guadalcázar, Count of Posadas | 25 July 1622 | 14 January 1629 |
| 14 | | Luis Jerónimo de Cabrera,
4th Count of Chinchón | 14 January 1629 | 18 December 1639 |
| 15 | | Pedro de Toledo,
1st Marquis of Mancera | 18 December 1639 | 20 September 1648 |
| 16 | | García Sarmiento de Sotomayor,
2nd Count of Salvatierra | 20 September 1648 | 24 February 1655 |
| 17 | | Luis Enríquez de Guzmán,
9th Count of Alba de Liste | 24 February 1655 | 31 December 1661 |
| 18 | | Diego de Benavides,
8th Count of Santisteban del Puerto, 1st Marquis of Solera | 31 December 1661 | 16 March 1666 |
| | Bernardo de Iturriaza,
Dean of the Audiencia | March 1666 | November 1667 |
Charles II |
| 19 | | Pedro Antonio Fernández de Castro,
10th Count of Lemos | 21 November 1667 | 6 December 1672 |
| | Bernardo de Iturriaza,
Dean of the Audiencia | December 1672 | August 1674 |
| 20 | | Baltasar de la Cueva,
Count of Castellar | 15 August 1674 | 7 July 1678 |
| 21 | | Melchor Liñán y Cisneros
(Archbishop of Lima) | 7 July 1678 | 20 November 1681 |
| 22 | | Melchor de Navarra,
Duke of Palata | 20 November 1681 | 15 August 1689 |
| 23 | | Melchor Portocarrero,
3rd Count of Monclova | August 1689 | September 1705 |
| | Juan Peñalosa y Benavides,
Dean of the Audiencia | September 1705 | May 1707 |
Philip V |
| 24 | | Manuel de Oms,
1st Marquis of Castelldosrius | 7 July 1707 | 22 April 1710 |
| | Miguel Núñez de Sanabria,
Dean of the Audiencia | 22 April 1710 | 14 September 1710 |
| 25 | | Diego Ladrón de Guevara | 14 September 1710 | 2 March 1716 |
| | Mateo de la Mata Ponce de León,
Dean of the Audiencia | 2 March 1716 | 15 August 1716 |
| | Diego Morcillo Rubio de Auñón,
(Archbishop of Lima) | 15 August 1716 | 5 October 1716 |
| 26 | | Carmine Caracciolo,
5th Prince of Santo Buono | 5 October 1716 | 26 January 1720 |
| 27 | | Diego Morcillo Rubio de Auñón,
(Archbishop of Lima) | 26 January 1720 | 14 May 1724 |
| 28 | | José de Armendáriz,
1st Marquis of Castelfuerte | 14 May 1724 | 4 February 1736 |
Louis I
|
| 29 | | José Antonio de Mendoza,
3rd Marquis of Villagarcía | 4 February 1736 | 15 December 1745 |
Philip V |
| 30 | | José Manso de Velasco,
1st Count of Superunda | 15 December 1745 | 12 October 1761 |
Ferdinand VI |
| 31 | | Manuel de Amat y Juniet | 12 October 1761 | 17 July 1776 |
Charles III |
| 32 | | Manuel de Guirior | 17 July 1776 | 21 July 1780 |
| 33 | | Agustín de Jáuregui | 21 July 1780 | 29 April 1784 |
| 34 | | Teodoro de Croix | 29 April 1784 | 25 March 1790 |
| 35 | | Francisco Gil de Taboada | 25 March 1790 | 6 June 1796 |
Charles IV |
| 36 | | Ambrosio O'Higgins,
1st Marquis of Osorno | 24 July 1796 | 19 March 1801 |
| | Manuel Arredondo y Pelegrín,
Dean of the Audiencia | 19 March 1801 | 5 November 1801 |
| 37 | | Gabriel de Avilés,
2nd Marquis of Avilés | 5 November 1801 | 20 August 1806 |
| 38 | | José Fernando de Abascal y Sousa,
1st Marquis of La Concordia | 20 August 1806 | 7 July 1816 |
| 39 | | Joaquín de la Pezuela,
1st Marquis of Viluma | 7 July 1816 | 29 January 1821 |
Ferdinand VII |
| 40 | | José de la Serna e Hinojosa,
1st Count of los Andes | 29 January 1821 | 9 December 1824 |
| 41 | | Juan Pío de Tristán y Moscoso | 9 December 1824 | 30 December 1824[1] | |
