VPS29 explained

VPS29 is a human gene coding for the vacuolar protein sorting protein Vps29, a component of the retromer complex.^[1]

Yeast homolog

The homologous protein (one that performs the same function) in yeast is Vacuolar protein sorting 29 homolog (S. cerevisiae).^[2]

Function

VPS29 belongs to a group of genes coding for vacuolar protein sorting (VPS) proteins that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases.^[3] The protein encoded by this gene, Vps29, is a component of a large multimeric complex, termed the retromer complex, which is involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Vps29 may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment.^[4] Alternative splice variants encoding different isoforms, and usage of multiple polyadenylation sites have been found for this gene.

Further reading

• Seaman MN, McCaffery JM, Emr SD . A Membrane Coat Complex Essential for Endosome-to-Golgi Retrograde Transport in Yeast . J. Cell Biol. . 142 . 3 . 665–81 . 1998 . 9700157 . 10.1083/jcb.142.3.665 . 2148169 .
• Edgar AJ, Polak JM . Human homologues of yeast vacuolar protein sorting 29 and 35 . Biochem. Biophys. Res. Commun. . 277 . 3 . 622–30 . 2000 . 11062004 . 10.1006/bbrc.2000.3727 .
• Hartley JL, Temple GF, Brasch MA . DNA Cloning Using In Vitro Site-Specific Recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Haft CR . Human Orthologs of Yeast Vacuolar Protein Sorting Proteins Vps26, 29, and 35: Assembly into Multimeric Complexes . Mol. Biol. Cell . 11 . 12 . 4105–16 . 2001 . 11102511 . 10.1091/mbc.11.12.4105. 15060 . vanc. de la Luz Sierra M . Bafford R . 3 . Lesniak . MA . Barr . VA . Taylor . SI .
• Wiemann S . Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 . vanc. Weil B . Wellenreuther R . 3 . Gassenhuber . J . Glassl . S . Ansorge . W . Böcher . M . Blöcker . H . Bauersachs . S .
• Simpson JC . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Rep. . 1 . 3 . 287–92 . 2001 . 11256614 . 10.1093/embo-reports/kvd058 . 1083732 . vanc. Wellenreuther R . Poustka A . 3 . Pepperkok . R . Wiemann . S .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Vergés M . The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor . Nat. Cell Biol. . 6 . 8 . 763–9 . 2004 . 15247922 . 10.1038/ncb1153 . vanc. Luton F . Gruber C . 3 . Tiemann . Frank . Reinders . Lorri G. . Huang . Lan . Burlingame . Alma L. . Haft . Carol R. . Mostov . Keith E. . 22296469 .
• Mingot JM, Bohnsack MT, Jäkle U, Görlich D . Exportin 7 defines a novel general nuclear export pathway . EMBO J. . 23 . 16 . 3227–36 . 2005 . 15282546 . 10.1038/sj.emboj.7600338 . 514512 .
• Gerhard DS . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Wiemann S . From ORFeome to Biology: A Functional Genomics Pipeline . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . vanc. Arlt D . Huber W . 3 . Wellenreuther . R . Schleeger . S . Mehrle . A . Bechtel . S . Sauermann . M . Korf . U .
• Wang D . Crystal structure of human vacuolar protein sorting protein 29 reveals a phosphodiesterase/nuclease-like fold and two protein-protein interaction sites . J. Biol. Chem. . 280 . 24 . 22962–7 . 2005 . 15788412 . 10.1074/jbc.M500464200 . vanc. Guo M . Liang Z . 3 . Fan . J . Zhu . Z . Zang . J . Zhu . Z . Li . X . Teng . M . free .
• Mehrle A . The LIFEdb database in 2006 . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 . vanc. Rosenfelder H . Schupp I . 3 . Del Val . C . Arlt . D . Hahne . F . Bechtel . S . Simpson . J . Hofmann . O .
• Damen E . The human Vps29 retromer component is a metallo-phosphoesterase for a cation-independent mannose 6-phosphate receptor substrate peptide . Biochem. J. . 398 . 3 . 399–409 . 2006 . 16737443 . 10.1042/BJ20060033 . 1559457 . vanc. Krieger E . Nielsen JE . 3 . Eygensteyn . Jelle . Van Leeuwen . Jeroen E. M. .
• Rojas R, Kametaka S, Haft CR, Bonifacino JS . Interchangeable but Essential Functions of SNX1 and SNX2 in the Association of Retromer with Endosomes and the Trafficking of Mannose 6-Phosphate Receptors . Mol. Cell. Biol. . 27 . 3 . 1112–24 . 2007 . 17101778 . 10.1128/MCB.00156-06 . 1800681 .
• Hierro A . Functional architecture of the retromer cargo-recognition complex . Nature . 449 . 7165 . 1063–7 . 2007 . 17891154 . 10.1038/nature06216 . 2377034 . vanc. Rojas AL . Rojas R . 3 . Murthy . Namita . Effantin . Grégory . Kajava . Andrey V. . Steven . Alasdair C. . Bonifacino . Juan S. . Hurley . James H. . 2007Natur.449.1063H .

Notes and References

1. Edgar AJ, Polak JM . Human homologues of yeast vacuolar protein sorting 29 and 35 . Biochem. Biophys. Res. Commun. . 277 . 3 . 622–30 . November 2000 . 11062004 . 10.1006/bbrc.2000.3727 .
2. Web site: Entrez Gene: VPS29 vacuolar protein sorting 29 homolog (S. cerevisiae).
3. Haft CR, de la Luz Sierra M, Bafford R, Lesniak MA, Barr VA, Taylor SI . Human Orthologs of Yeast Vacuolar Protein Sorting Proteins Vps26, 29, and 35: Assembly into Multimeric Complexes . Mol. Biol. Cell . 11 . 12 . 4105–16 . December 2000 . 11102511 . 15060 . 10.1091/mbc.11.12.4105.
4. Bonifacino JS, Hurley JH . Retromer . Curr. Opin. Cell Biol. . 20 . 4 . 427–36 . August 2008 . 18472259 . 10.1016/j.ceb.2008.03.009 . 2833274 .