VPS28 explained

Vacuolar protein sorting-associated protein 28 homolog is a protein that in humans is encoded by the VPS28 gene.^[1]

Function

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated protein. The two other subunits of ESCRT-I are vacuolar protein sorting 23 (VPS23), also known as tumor susceptibility gene 101 (TSG101), and vacuolar protein sorting 37 (VPS37). Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

Interactions

VPS28 has been shown to interact with TSG101.^{[2] [3] [4]}

Further reading

• Raiborg C, Rusten TE, Stenmark H . Protein sorting into multivesicular endosomes . Curr. Opin. Cell Biol. . 15 . 4 . 446–55 . 2004 . 12892785 . 10.1016/S0955-0674(03)00080-2 .
• Bishop N, Woodman P . TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes . J. Biol. Chem. . 276 . 15 . 11735–42 . 2001 . 11134028 . 10.1074/jbc.M009863200 . free .
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y . Protein–Protein Interaction Panel Using Mouse Full-Length cDNAs . Genome Res. . 11 . 10 . 1758–65 . 2001 . 11591653 . 311163 . 10.1101/gr.180101 .
• Bishop N, Horman A, Woodman P . Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein–ubiquitin conjugates . J. Cell Biol. . 157 . 1 . 91–101 . 2002 . 11916981 . 2173266 . 10.1083/jcb.200112080 .
• . Role of ESCRT-I in Retroviral Budding . J. Virol. . 77 . 8 . 4794–804 . 2003 . 12663786 . 152150 . 10.1128/JVI.77.8.4794-4804.2003 .
• Tanzi GO, Piefer AJ, Bates P . Equine Infectious Anemia Virus Utilizes Host Vesicular Protein Sorting Machinery during Particle Release . J. Virol. . 77 . 15 . 8440–7 . 2003 . 12857913 . 165230 . 10.1128/JVI.77.15.8440-8447.2003 .
• Martin-Serrano J, Yarovoy A, Perez-Caballero D, Bieniasz PD, Yaravoy A . Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins . Proc. Natl. Acad. Sci. U.S.A. . 100 . 21 . 12414–9 . 2003 . 14519844 . 218772 . 10.1073/pnas.2133846100 . 2003PNAS..10012414M . free .
• Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI . The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding . J. Biol. Chem. . 279 . 34 . 36059–71 . 2005 . 15218037 . 10.1074/jbc.M405226200 . free .
• Bache KG, Slagsvold T, Cabezas A, Rosendal KR, Raiborg C, Stenmark H . The Growth-Regulatory Protein HCRP1/hVps37A Is a Subunit of Mammalian ESCRT-I and Mediates Receptor Down-Regulation . Mol. Biol. Cell . 15 . 9 . 4337–46 . 2005 . 15240819 . 515363 . 10.1091/mbc.E04-03-0250 .
• Eastman SW, Martin-Serrano J, Chung W, Zang T, Bieniasz PD . Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding . J. Biol. Chem. . 280 . 1 . 628–36 . 2005 . 15509564 . 10.1074/jbc.M410384200 . free.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Web site: Entrez Gene: VPS28 vacuolar protein sorting 28 homolog (S. cerevisiae).
2. Huda Zoghbi . Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
3. Stuchell MD, Garrus JE, Müller B, Stray KM, Ghaffarian S, McKinnon R, Kräusslich HG, Morham SG, Sundquist WI . The human endosomal sorting complex required for transport (ESCRT-I) and its role in HIV-1 budding . J. Biol. Chem. . 279 . 34 . 36059–71 . August 2004 . 15218037 . 10.1074/jbc.M405226200 . free .
4. Bishop N, Woodman P . TSG101/mammalian VPS23 and mammalian VPS28 interact directly and are recruited to VPS4-induced endosomes . J. Biol. Chem. . 276 . 15 . 11735–42 . April 2001 . 11134028 . 10.1074/jbc.M009863200 . free .