Unsaturated rhamnogalacturonyl hydrolase explained

Unsaturated rhamnogalacturonyl hydrolase
Ec Number:	3.2.1.172

Unsaturated rhamnogalacturonyl hydrolase (YteR, YesR) is an enzyme with systematic name 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose hydrolase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H₂O

\rightleftharpoons 5-dehydro-4-deoxy-D-glucuronate + L-rhamnopyranose

The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168.

Notes and References

1. Itoh T, Ochiai A, Mikami B, Hashimoto W, Murata K . A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide . Journal of Molecular Biology . 360 . 3 . 573–85 . July 2006 . 16781735 . 10.1016/j.jmb.2006.04.047 .
2. Zhang R, Minh T, Lezondra L, Korolev S, Moy SF, Collart F, Joachimiak A . 1.6 A crystal structure of YteR protein from Bacillus subtilis, a predicted lyase . Proteins . 60 . 3 . 561–5 . August 2005 . 15906318 . 2792013 . 10.1002/prot.20410 .
3. Itoh T, Ochiai A, Mikami B, Hashimoto W, Murata K . Structure of unsaturated rhamnogalacturonyl hydrolase complexed with substrate . Biochemical and Biophysical Research Communications . 347 . 4 . 1021–9 . September 2006 . 16870154 . 10.1016/j.bbrc.2006.07.034 .