Unsaturated chondroitin disaccharide hydrolase explained

Unsaturated chondroitin disaccharide hydrolase
Ec Number:	3.2.1.180

Unsaturated chondroitin disaccharide hydrolase (UGL, unsaturated glucuronyl hydrolase) is an enzyme with systematic name beta-D-4-deoxy-Delta4-GlcAp-(1->3)-beta-D-GalNAc6S hydrolase.^{[1] [2]} This enzyme catalyses the following chemical reaction

beta-D-4-deoxy-Delta4-GlcAp-(1->3)-beta-D-GalNAc6S + H₂O

\rightleftharpoons 5-dehydro-4-deoxy-D-glucuronate + N-acetyl-beta-D-galactosamine-6-O-sulfate

The enzyme releases 4-deoxy-4,5-didehydro D-glucuronic acid or 4-deoxy-4,5-didehydro L-iduronic acid from chondroitin disaccharides, hyaluronan disaccharides and heparin disaccharides.

Notes and References

1. Maruyama Y, Nakamichi Y, Itoh T, Mikami B, Hashimoto W, Murata K . Substrate specificity of streptococcal unsaturated glucuronyl hydrolases for sulfated glycosaminoglycan . J. Biol. Chem. . 284 . 27 . 18059–69 . July 2009 . 19416976 . 2709336 . 10.1074/jbc.M109.005660. free .
2. Nakamichi Y, Maruyama Y, Mikami B, Hashimoto W, Murata K . Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups . J. Biol. Chem. . 286 . 8 . 6262–71 . February 2011 . 21147778 . 3057837 . 10.1074/jbc.M110.182618 . free .