University of Tartu Botanical Garden (Estonian: Tartu Ülikooli Botaanikaaed), is a botanical garden in Tartu, Estonia. It belongs to the University of Tartu.
The Garden was established in 1803. Originally, it was located at Vanemuise street near what is now the Vanemuine Small Theatre House. In 1806, the garden was relocated to a more suitable site, on a former bastion at Lai street on the northern side of the old town.