| Election Name: | 2000 United States Senate election in Minnesota |
| Country: | Minnesota |
| Flag Year: | 1983 |
| Type: | presidential |
| Ongoing: | no |
| Previous Election: | 1994 United States Senate election in Minnesota |
| Previous Year: | 1994 |
| Next Election: | 2006 United States Senate election in Minnesota |
| Next Year: | 2006 |
| Election Date: | November 7, 2000 |
| Image1: | Mark Dayton official photo.jpg |
| Nominee1: | Mark Dayton |
| Party1: | Democratic–Farmer–Labor Party (Minnesota) |
| Popular Vote1: | 1,181,553 |
| Percentage1: | 48.83% |
| Party2: | Republican Party (US) |
| Popular Vote2: | 1,047,474 |
| Percentage2: | 43.29% |
| Image3: | 3x4.svg |
| Party3: | Independence Party of Minnesota |
| Popular Vote3: | 140,583 |
| Percentage3: | 5.81% |
| Map Size: | 270px |
| U.S. Senator | |
| Before Party: | Republican Party (US) |
| After Party: | Democratic–Farmer–Labor Party (Minnesota) |
The 2000 United States Senate election in Minnesota was held on November 7, 2000, to select a U.S. senator from the state of Minnesota. The race pitted incumbent Republican Senator Rod Grams against former Minnesota State Auditor Mark Dayton. Dayton won with 48.83% of the vote to Grams's 43.29%. Dayton declined to run for reelection in 2006 and ran successfully in 2010 and 2014 for governor of Minnesota. He was succeeded in the Senate by Amy Klobuchar, who has held the seat ever since. Upon Dayton's swearing in, Democrats held both of Minnesota's U.S. Senate seats for the first time since 1978. As of, this is the last time that a man won the Class 1 Senate seat in Minnesota.
Dayton and Grams had three debates, one on October 18, one on October 26, and one on November 3.