| Election Name: | 1964 United States Senate election in Florida |
| Country: | Florida |
| Flag Year: | 1900 |
| Type: | presidential |
| Ongoing: | no |
| Previous Election: | 1958 United States Senate election in Florida |
| Previous Year: | 1958 |
| Next Election: | 1970 United States Senate election in Florida |
| Next Year: | 1970 |
| Election Date: | November 3, 1964 |
| Image1: | Spessard Holland.JPG |
| Nominee1: | Spessard Holland |
| Party1: | Democratic Party (United States) |
| Popular Vote1: | 997,585 |
| Percentage1: | 63.93% |
| Party2: | Republican Party (United States) |
| Popular Vote2: | 562,212 |
| Percentage2: | 36.03% |
| U.S. Senator | |
| Map Size: | 280px |
| Before Party: | Democratic Party (US) |
| After Party: | Democratic Party (US) |
The 1964 United States Senate election in Florida was held on November 3, 1964. Incumbent Democratic U.S. Senator Spessard Holland was re-elected to a fourth term in office, defeating J. Brailey Oldham in the primary and Republican Claude R. Kirk Jr. in the general election.