| Election Name: | 1916 United States Senate election in Florida |
| Country: | Florida |
| Type: | presidential |
| Ongoing: | no |
| Previous Election: | 1911 United States Senate election in Florida |
| Previous Year: | 1911 |
| Next Election: | 1922 United States Senate election in Florida |
| Next Year: | 1922 |
| Election Date: | November 7, 1916 |
| Image1: | Park Trammell.jpg |
| Nominee1: | Park Trammell |
| Party1: | Democratic Party (United States) |
| Popular Vote1: | 58,391 |
| Percentage1: | 82.86% |
| Nominee2: | William R. O'Neal |
| Party2: | Republican Party (United States) |
| Popular Vote2: | 8,774 |
| Percentage2: | 12.45% |
| Map Size: | 301px |
| U.S. Senator | |
| Before Election: | Nathan Philemon Bryan |
| Before Party: | Democratic Party (US) |
| After Election: | Park Trammell |
| After Party: | Democratic Party (US) |
| Flag Year: | 1900 |
The 1916 United States Senate election in Florida was held on November 7, 1916.
Incumbent Democratic Senator Nathan Philemon Bryan ran for re-election to a second term in office, but lost the Democratic nomination to Governor of Florida Park Trammell. Trammell easily won the general election.