| Post: | Ambassador |
| Body: | the United States to São Tomé and Príncipe |
| Insignia: | U.S. Department of State official seal.svg |
| Insigniasize: | 120px |
| Insigniacaption: | Seal of the United States Department of State |
| Incumbent: | Tulinabo S. Mushingi |
| Incumbentsince: | August 10, 2022 |
| Nominator: | The President of the United States |
| Inaugural: | Andrew L. Steigman as Ambassador Extraordinary and Plenipotentiary |
| Formation: | December 11, 1975 |
| Website: | U.S. Embassy - Luanda |
The United States ambassador to São Tomé and Príncipe is the official representative of the government of the United States to the government of São Tomé and Príncipe. Until 2022, the ambassador was concurrently the ambassador to Gabon, while resident in Libreville. Beginning in 2022, representation for São Tomé and Príncipe was moved to Luanda, with the ambassador concurrently the ambassador to Angola, due to the countries' "longstanding cultural, linguistic, and economic ties."[1]
| Andrew L. Steigman[2] – Career FSO | Ambassador Extraordinary and Plenipotentiary | December 11, 1975 | February 14, 1976 | August 21, 1977 | ||
| Arthur T. Tienken – Career FSO | Ambassador Extraordinary and Plenipotentiary | February 2, 1978 | May 26, 1978 | July 19, 1981 | ||
| Francis Terry McNamara – Career FSO | Ambassador Extraordinary and Plenipotentiary | December 11, 1981 | July 26, 1982 | August 3, 1984 | ||
| Larry C. Williamson – Career FSO | Ambassador Extraordinary and Plenipotentiary | August 13, 1984 | October 29, 1984 | August 21, 1987 | ||
| Warren Clark Jr. – Career FSO | Ambassador Extraordinary and Plenipotentiary | August 10, 1987 | October 19, 1987 | August 24, 1989 | ||
| Keith Leveret Wauchope – Career FSO | Ambassador Extraordinary and Plenipotentiary | November 6, 1989 | February 5, 1990 | July 13, 1992 | ||
| Joseph Charles Wilson IV – Career FSO | Ambassador Extraordinary and Plenipotentiary | July 14, 1992 | September 29, 1992 | August 4, 1995 | ||
| Elizabeth Raspolic – Career FSO | Ambassador Extraordinary and Plenipotentiary | October 3, 1995 | November 10, 1995 | July 24, 1998 | ||
| James V. Ledesma – Career FSO | Ambassador Extraordinary and Plenipotentiary | October 22, 1998 | January 27, 1999 | June 18, 2001 | The post was vacant June 2001–June 2002. Thomas F. Daughton served as Chargé d'Affaires ad interim in the interval. | |
| Kenneth Price Moorfield – Career FSO | Ambassador Extraordinary and Plenipotentiary | January 30, 2002 | June 20, 2002 | July 31, 2004 | ||
| R. Barrie Walkley – Career FSO | Ambassador Extraordinary and Plenipotentiary | July 2, 2004 | November 11, 2004 | April 27, 2007 | ||
| Eunice S. Reddick – Career FSO | Ambassador Extraordinary and Plenipotentiary | October 29, 2007 | December 6, 2007 | July 18, 2010 | ||
| Eric D. Benjaminson – Career FSO | Ambassador Extraordinary and Plenipotentiary | September 15, 2010 | December 3, 2010 | May 21, 2013 | ||
| Cynthia Akuetteh – Career FSO | Ambassador Extraordinary and Plenipotentiary | August 1, 2014 | April 10, 2015 | February 26, 2018[3] | ||
| Joel Danies – Career FSO | Ambassador Extraordinary and Plenipotentiary | February 21, 2018 | April 20, 2018 | March 1, 2019 | ||
| Robert E. Whitehead – Career FSO | Chargé d’Affaires a.i. | March 1, 2019 | August 17, 2020 | |||
| Tulinabo S. Mushingi | Ambassador Extraordinary and Plenipotentiary | December 18, 2021 | August 10, 2022 |