| State: | KY |
| Type: | US |
| Route: | 27 |
| Map Custom: | yes |
| Map Notes: | US 27 highlighted in red |
| Length Mi: | 201.120 |
| Length Ref: | [1] |
| Established: | [2] [3] |
| Direction A: | South |
| Terminus A: | at Tennessee state line near Strunk |
| Junction: |
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| Direction B: | North |
| Terminus B: | at Ohio state line in Covington |
| Counties: | McCreary, Pulaski, Lincoln, Garrard, Jessamine, Fayette, Bourbon, Harrison, Pendleton, Campbell |
| Previous Type: | KY |
| Previous Route: | 26 |
| Next Type: | KY |
| Next Route: | 28 |
U.S. Route 27 (US 27) in Kentucky runs 201.12miles from the Tennessee border to the Ohio border at Cincinnati. It crosses into the state in the Lake Cumberland area, passing near or through many small towns, including Somerset, Stanford, and Nicholasville. The road then passes straight through the heart of Lexington, including past the University of Kentucky (UK) and Transylvania University. North of Lexington, it passes through Cynthiana and Falmouth before entering Campbell County and passing through many Northern Kentucky suburbs before ending at the Ohio state line on the Taylor–Southgate Bridge in Cincinnati.
US 27 crosses into Kentucky entering the Daniel Boone National Forest in the Strunk community, just south of Pine Knot. Heading north, US 27 turns into a four-lane highway in Burnside. US 27 is the main highway running through Somerset. The route continues as a four-lane highway almost to the Pulaski–Lincoln county line and again has four lanes through Stanford. It veers northeast to Lancaster then northwest to the site of Camp Dick Robinson at Kentucky Route 34, becoming four lanes. Entering Jessamine County, the road crosses the Kentucky River. It westerly bypasses the central business district of Nicholasville. Once in Fayette County, US 27 turns into a six-lane highway passing many shopping destinations in Lexington. Passing The Summit at Fritz Farm, Fayette Mall, and Lexington Green, Nicholasville Road is one of the busiest roads in Lexington. The road becomes South Limestone Street upon reaching Cooper Drive. At UK's Albert B. Chandler Hospital, it turns west on four-lane Virginia Avenue, then joins US 68 (Broadway) for its northeasterly journey through Lexington's central business district and the horse-farm country of Fayette and Bourbon counties. The conjoined routes separate on the north side of Paris, where US 27 splits from the town bypass and heads north to Cynthiana, Falmouth, and Newport. It then crosses the Ohio River into Cincinnati.
