USP16 explained

Ubiquitin carboxyl-terminal hydrolase 16 is an enzyme that in humans is encoded by the USP16 gene.^{[1] [2]}

This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Further reading

• D'Andrea A, Pellman D . Deubiquitinating enzymes: a new class of biological regulators . Crit. Rev. Biochem. Mol. Biol. . 33 . 5 . 337–52 . 1999 . 9827704 . 10.1080/10409239891204251 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Cai SY, Babbitt RW, Marchesi VT . A mutant deubiquitinating enzyme (Ubp-M) associates with mitotic chromosomes and blocks cell division . Proc. Natl. Acad. Sci. U.S.A. . 96 . 6 . 2828–33 . 1999 . 10077596 . 10.1073/pnas.96.6.2828 . 15854 . 1999PNAS...96.2828C . free .
• Hattori M, Fujiyama A, Taylor TD, etal . The DNA sequence of human chromosome 21 . Nature . 405 . 6784 . 311–9 . 2000 . 10830953 . 10.1038/35012518 . 2000Natur.405..311H . free .
• Wistow G, Bernstein SL, Wyatt MK, etal . Expressed sequence tag analysis of human retina for the NEIBank Project: retbindin, an abundant, novel retinal cDNA and alternative splicing of other retina-preferred gene transcripts . Mol. Vis. . 8 . 196–204 . 2002 . 12107411 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Lehner B, Sanderson CM . A protein interaction framework for human mRNA degradation . Genome Res. . 14 . 7 . 1315–23 . 2004 . 15231747 . 10.1101/gr.2122004 . 442147 .
• Beausoleil SA, Jedrychowski M, Schwartz D, etal . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . free .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Rual JF, Venkatesan K, Hao T, etal . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Pai MT, Tzeng SR, Kovacs JJ, etal . Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin . J. Mol. Biol. . 370 . 2 . 290–302 . 2007 . 17512543 . 10.1016/j.jmb.2007.04.015 . 2870993 .
• Joo HY, Zhai L, Yang C, etal . Regulation of cell cycle progression and gene expression by H2A deubiquitination . Nature . 449 . 7165 . 1068–72 . 2007 . 17914355 . 10.1038/nature06256 . 2007Natur.449.1068J . 4417239 .

Notes and References

1. Puente XS, Sanchez LM, Overall CM, Lopez-Otin C . Human and mouse proteases: a comparative genomic approach . Nat Rev Genet . 4 . 7 . 544–58 . Jul 2003 . 12838346 . 10.1038/nrg1111 . 2856065 .
2. Web site: Entrez Gene: USP16 ubiquitin specific peptidase 16.