USP14 explained

Ubiquitin-specific protease 14 is an enzyme that in humans is encoded by the USP14 gene.^{[1] [2]}

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.^[2]

Interactions

USP14 has been shown to interact with CXCR4.^[3]

Further reading

• Wilson SM, Bhattacharyya B, Rachel RA, Coppola V, Tessarollo L, Householder DB, Fletcher CF, Miller RJ, Copeland NG, Jenkins NA . Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease. . Nature Genetics . 32 . 420–425 . 2002 . 12368914 . 10.1038/ng1006 . 3. 23542012 .
• D'Andrea A, Pellman D . Deubiquitinating enzymes: a new class of biological regulators. . Crit. Rev. Biochem. Mol. Biol. . 33 . 5 . 337–52 . 1999 . 9827704 . 10.1080/10409239891204251 .
• Deshpande KL, Seubert PH, Tillman DM, Farkas WR, Katze JR . Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit. . Arch. Biochem. Biophys. . 326 . 1 . 1–7 . 1996 . 8579355 . 10.1006/abbi.1996.0039 .
• Reuter TY, Medhurst AL, Waisfisz Q, Zhi Y, Herterich S, Hoehn H, Gross HJ, Joenje H, Hoatlin ME, Mathew CG, Huber PA . Yeast two-hybrid screens imply involvement of Fanconi anemia proteins in transcription regulation, cell signaling, oxidative metabolism, and cellular transport. . Exp. Cell Res. . 289 . 2 . 211–21 . 2003 . 14499622 . 10.1016/S0014-4827(03)00261-1 .
• Dennehey BK, Gutches DG, McConkey EH, Krauter KS . Inversion, duplication, and changes in gene context are associated with human chromosome 18 evolution. . Genomics . 83 . 3 . 493–501 . 2004 . 14962675 . 10.1016/j.ygeno.2003.08.017 .
• Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y . Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14. . EMBO J. . 24 . 21 . 3747–56 . 2005 . 16211010 . 1276716 . 10.1038/sj.emboj.7600832 .
• Shinji S, Naito Z, Ishiwata S, Ishiwata T, Tanaka N, Furukawa K, Suzuki H, Seya T, Matsuda A, Katsuta M, Tajiri T . Ubiquitin-specific protease 14 expression in colorectal cancer is associated with liver and lymph node metastases. . Oncol. Rep. . 15 . 3 . 539–43 . 2006 . 16465409 . 10.3892/or.15.3.539 . free .
• Crimmins S, Jin Y, Wheeler C, Huffman AK, Chapman C, Dobrunz LE, Levey A, Roth KA, Wilson JA, Wilson SM . Transgenic rescue of ataxia mice with neuronal-specific expression of ubiquitin-specific protease 14. . J. Neurosci. . 26 . 44 . 11423–31 . 2006 . 17079671 . 10.1523/JNEUROSCI.3600-06.2006 . 6674543 . free .

Notes and References

1. Puente XS, Sánchez LM, Overall CM, López-Otín C . Human and mouse proteases: a comparative genomic approach . Nat Rev Genet . 4 . 7 . 544–58 . Jul 2003 . 12838346 . 10.1038/nrg1111 . 2856065 .
2. Web site: Entrez Gene: USP14 ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase).
3. Mines MA, Goodwin JS, Limbird LE, Cui FF, Fan GH . Deubiquitination of CXCR4 by USP14 is critical for both CXCL12-induced CXCR4 degradation and chemotaxis but not ERK activation . J. Biol. Chem. . 284 . 9 . 5742–52 . Feb 2009 . 19106094 . 2645827 . 10.1074/jbc.M808507200 . free .