USO1 explained

General vesicular transport factor p115 is a protein that in humans is encoded by the USO1 gene.^{[1] [2] [3] [4] [5] [6]}

Function

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum (ER), where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GOLGA2, and giantin in a sequential manner.

Interactions

USO1 has been shown to interact with:

• GOSR1,^[7]
• GOSR2,^{[7] [8]}
• SCFD1,^{[7] [8]} and
• STX5.^{[7] [8]}

Further reading

• Waters MG, Clary DO, Rothman JE . A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack . J. Cell Biol. . 118 . 5 . 1015–26 . 1992 . 1512287 . 2289595 . 10.1083/jcb.118.5.1015 .
• Barr FA, Nakamura N, Warren G . Mapping the interaction between GRASP65 and GM130, components of a protein complex involved in the stacking of Golgi cisternae . EMBO J. . 17 . 12 . 3258–68 . 1998 . 9628863 . 1170664 . 10.1093/emboj/17.12.3258 .
• Nelson DS, Alvarez C, Gao YS, García-Mata R, Fialkowski E, Sztul E . The membrane transport factor TAP/p115 cycles between the Golgi and earlier secretory compartments and contains distinct domains required for its localization and function . J. Cell Biol. . 143 . 2 . 319–31 . 1998 . 9786945 . 2132831 . 10.1083/jcb.143.2.319 .
• Allan BB, Moyer BD, Balch WE . Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion . Science . 289 . 5478 . 444–8 . 2000 . 10903204 . 10.1126/science.289.5478.444 . 2000Sci...289..444A .
• Dirac-Svejstrup AB, Shorter J, Waters MG, Warren G . Phosphorylation of the vesicle-tethering protein p115 by a casein kinase II-like enzyme is required for Golgi reassembly from isolated mitotic fragments . J. Cell Biol. . 150 . 3 . 475–88 . 2000 . 10931861 . 2175190 . 10.1083/jcb.150.3.475 .
• Alvarez C, Garcia-Mata R, Hauri HP, Sztul E . The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic . J. Biol. Chem. . 276 . 4 . 2693–700 . 2001 . 11035033 . 10.1074/jbc.M007957200 . free .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 310948 . 10.1101/gr.143000 .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Brunati AM, Marin O, Folda A, Meggio F, Pinna LA . Possible implication of the Golgi apparatus casein kinase in the phosphorylation of vesicle docking protein p115 Ser-940: a study with peptide substrates . Biochem. Biophys. Res. Commun. . 284 . 3 . 817–22 . 2001 . 11396975 . 10.1006/bbrc.2001.5049 .
• Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G . Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 . J. Cell Biol. . 157 . 1 . 45–62 . 2002 . 11927603 . 2173270 . 10.1083/jcb.200112127 .
• Short B, Preisinger C, Schaletzky J, Kopajtich R, Barr FA . The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes . Curr. Biol. . 12 . 20 . 1792–5 . 2003 . 12401177 . 10.1016/S0960-9822(02)01221-6 . 14031165 . free .
• Chiu R, Novikov L, Mukherjee S, Shields D . A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis . J. Cell Biol. . 159 . 4 . 637–48 . 2003 . 12438416 . 2173109 . 10.1083/jcb.200208013 .
• Han SJ, Lee JH, Kim CG, Hong SH . Identification of p115 as a PLCgamma1-binding protein and the role of Src homology domains of PLCgamma1 in the vesicular transport . Biochem. Biophys. Res. Commun. . 300 . 3 . 649–55 . 2003 . 12507498 . 10.1016/S0006-291X(02)02884-X .
• García-Mata R, Sztul E . The membrane-tethering protein p115 interacts with GBF1, an ARF guanine-nucleotide-exchange factor . EMBO Rep. . 4 . 3 . 320–5 . 2003 . 12634853 . 1315890 . 10.1038/sj.embor.embor762 .

Notes and References

1. Sohda M, Misumi Y, Yano A, Takami N, Ikehara Y . Phosphorylation of the vesicle docking protein p115 regulates its association with the Golgi membrane . J Biol Chem . 273 . 9 . 5385–8 . March 1998 . 9478999 . 10.1074/jbc.273.9.5385 . free .
2. Nakamura N, Lowe M, Levine TP, Rabouille C, Warren G . The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner . Cell . 89 . 3 . 445–55 . June 1997 . 9150144 . 10.1016/S0092-8674(00)80225-1 . 2135774 . free .
3. Whyte JR, Munro S . Vesicle tethering complexes in membrane traffic . J Cell Sci . 115 . Pt 13 . 2627–37 . June 2002 . 10.1242/jcs.115.13.2627 . 12077354 .
4. Short B, Haas A, Barr FA . Golgins and GTPases, giving identity and structure to the Golgi apparatus . Biochim Biophys Acta . 1744 . 3 . 383–95 . June 2005 . 15979508 . 10.1016/j.bbamcr.2005.02.001 . free .
5. Puthenveedu MA, Linstedt AD . Gene replacement reveals that p115/SNARE interactions are essential for Golgi biogenesis . Proc Natl Acad Sci U S A . 101 . 5 . 1253–6 . February 2004 . 14736916 . 337039 . 10.1073/pnas.0306373101 . 2004PNAS..101.1253P . free .
6. Web site: Entrez Gene: VDP vesicle docking protein p115.
7. Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G . Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 . J. Cell Biol. . 157 . 1 . 45–62 . April 2002 . 11927603 . 2173270 . 10.1083/jcb.200112127 .
8. Allan BB, Moyer BD, Balch WE . Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion . Science . 289 . 5478 . 444–8 . July 2000 . 10903204 . 10.1126/science.289.5478.444. 2000Sci...289..444A .