UDP-N-acetylmuramoyl-L-alanine—D-glutamate ligase explained

UDP-N-acetylmuramoyl-L-alanine—D-glutamate ligase
Ec Number:	6.3.2.9
Cas Number:	9023-59-0
Go Code:	0008764

In enzymology, a UDP-N-acetylmuramoyl-L-alanine—D-glutamate ligase is an enzyme that catalyzes the chemical reaction

ATP + UDP-N-acetylmuramoyl-L-alanine + D-glutamate

\rightleftharpoons ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate

The 3 substrates of this enzyme are ATP, UDP-N-acetylmuramoyl-L-alanine, and D-glutamate, whereas its 3 products are ADP, phosphate, and UDP-N-acetylmuramoyl-L-alanyl-D-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase (ADP-forming). Other names in common use include MurD synthetase, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase, uridine diphospho-N-acetylmuramoylalanyl-D-glutamate synthetase, D-glutamate-adding enzyme, D-glutamate ligase, UDP-Mur-NAC-L-Ala:D-Glu ligase, UDP-N-acetylmuramoyl-L-alanine:glutamate ligase (ADP-forming), and UDP-N-acetylmuramoylalanine-D-glutamate ligase. This enzyme participates in d-glutamine and d-glutamate metabolism and peptidoglycan biosynthesis.

Structural studies

As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes,,,,,,,, and .

See also

• Muramyl ligase

References

• Ito, E . Strominger JL . 1962 . Enzymatic synthesis of the peptide in bacterial uridine nucleotides I. Enzymatic addition of L-alanine, D-glutamic acid, and L-lysine . J. Biol. Chem. . 237 . 2689 - 2695 .
• van Heijenoort J . Recent advances in the formation of the bacterial peptidoglycan monomer unit . Natural Product Reports . 18 . 5 . 503–19 . October 2001 . 11699883 . 10.1039/a804532a .