UBR5 explained

E3 ubiquitin-protein ligase UBR5 is an enzyme that in humans is encoded by the UBR5 gene.^{[1] [2] [3]}

Function

This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.^[3]

Interactions

UBR5 has been shown to interact with:

• CIB1,^[4]
• Karyopherin alpha 1,^[4]
• MAPK1,^[5] and
• TOPBP1.^[6]

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones . DNA Res. . 9 . 3 . 99–106 . 2003 . 12168954 . 10.1093/dnares/9.3.99 . free .
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Res. . 5 . 6 . 355–64 . 1999 . 10048485 . 10.1093/dnares/5.6.355 . free .
• Deo RC, Sonenberg N, Burley SK . X-ray structure of the human hyperplastic discs protein: An ortholog of the C-terminal domain of poly(A)-binding protein . Proc. Natl. Acad. Sci. U.S.A. . 98 . 8 . 4414–9 . 2001 . 11287654 . 31849 . 10.1073/pnas.071552198 . free .
• Honda Y, Tojo M, Matsuzaki K, Anan T, Matsumoto M, Ando M, Saya H, Nakao M . Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response . J. Biol. Chem. . 277 . 5 . 3599–605 . 2002 . 11714696 . 10.1074/jbc.M104347200 . free .
• Henderson MJ, Russell AJ, Hird S, Muñoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK . EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response . J. Biol. Chem. . 277 . 29 . 26468–78 . 2002 . 12011095 . 10.1074/jbc.M203527200 . free . 1885/64590 . free .
• Eblen ST, Kumar NV, Shah K, Henderson MJ, Watts CK, Shokat KM, Weber MJ . Identification of novel ERK2 substrates through use of an engineered kinase and ATP analogs . J. Biol. Chem. . 278 . 17 . 14926–35 . 2003 . 12594221 . 10.1074/jbc.M300485200 . free .
• Zhang C, Dowd DR, Staal A, Gu C, Lian JB, van Wijnen AJ, Stein GS, MacDonald PN . Nuclear coactivator-62 kDa/Ski-interacting protein is a nuclear matrix-associated coactivator that may couple vitamin D receptor-mediated transcription and RNA splicing . J. Biol. Chem. . 278 . 37 . 35325–36 . 2003 . 12840015 . 10.1074/jbc.M305191200 . free .
• Clancy JL, Henderson MJ, Russell AJ, Anderson DW, Bova RJ, Campbell IG, Choong DY, Macdonald GA, Mann GJ, Nolan T, Brady G, Olopade OI, Woollatt E, Davies MJ, Segara D, Hacker NF, Henshall SM, Sutherland RL, Watts CK . EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer . Oncogene . 22 . 32 . 5070–81 . 2003 . 12902990 . 10.1038/sj.onc.1206775 . 25909437 .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional Proteomics Mapping of a Human Signaling Pathway . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nat. Biotechnol. . 23 . 1 . 94–101 . 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .
• Kim JE, Tannenbaum SR, White FM . Global phosphoproteome of HT-29 human colon adenocarcinoma cells . J. Proteome Res. . 4 . 4 . 1339–46 . 2005 . 16083285 . 10.1021/pr050048h .
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R . Phosphoproteome analysis of the human mitotic spindle . Proc. Natl. Acad. Sci. U.S.A. . 103 . 14 . 5391–6 . 2006 . 16565220 . 1459365 . 10.1073/pnas.0507066103 . 2006PNAS..103.5391N . free .
• Henderson MJ, Munoz MA, Saunders DN, Clancy JL, Russell AJ, Williams B, Pappin D, Khanna KK, Jackson SP, Sutherland RL, Watts CK . EDD mediates DNA damage-induced activation of CHK2 . J. Biol. Chem. . 281 . 52 . 39990–40000 . 2007 . 17074762 . 10.1074/jbc.M602818200 . 25298222 . free .
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . free .
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1 . 89 . 2007 . 17353931 . 1847948 . 10.1038/msb4100134 .

External links

• Ubr5 : Protein Overview : UCSD-Nature Molecule Pages

Notes and References

1. Callaghan MJ, Russell AJ, Woollatt E, Sutherland GR, Sutherland RL, Watts CK . Grant Robert Sutherland . Identification of a human HECT family protein with homology to the Drosophila tumor suppressor gene hyperplastic discs . Oncogene . 17 . 26 . 3479–91 . Mar 1999 . 10030672 . 10.1038/sj.onc.1202249 . 19850921 .
2. Tasaki T, Mulder LC, Iwamatsu A, Lee MJ, Davydov IV, Varshavsky A, Muesing M, Kwon YT . A Family of Mammalian E3 Ubiquitin Ligases That Contain the UBR Box Motif and Recognize N-Degrons . Mol Cell Biol . 25 . 16 . 7120–36 . Aug 2005 . 16055722 . 1190250 . 10.1128/MCB.25.16.7120-7136.2005 .
3. Web site: Entrez Gene: EDD1 E3 ubiquitin protein ligase, HECT domain containing, 1.
4. Henderson MJ, Russell AJ, Hird S, Muñoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK . EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response . J. Biol. Chem. . 277 . 29 . 26468–78 . Jul 2002 . 12011095 . 10.1074/jbc.M203527200 . free . 1885/64590 . free .
5. Eblen ST, Kumar NV, Shah K, Henderson MJ, Watts CK, Shokat KM, Weber MJ . Identification of novel ERK2 substrates through use of an engineered kinase and ATP analogs . J. Biol. Chem. . 278 . 17 . 14926–35 . Apr 2003 . 12594221 . 10.1074/jbc.M300485200 . free .
6. Honda Y, Tojo M, Matsuzaki K, Anan T, Matsumoto M, Ando M, Saya H, Nakao M . Cooperation of HECT-domain ubiquitin ligase hHYD and DNA topoisomerase II-binding protein for DNA damage response . J. Biol. Chem. . 277 . 5 . 3599–605 . Feb 2002 . 11714696 . 10.1074/jbc.M104347200 . free .