UBE2B explained

Ubiquitin-conjugating enzyme E2 B is a protein that in humans is encoded by the UBE2B gene.^{[1] [2]}

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.^[2]

Interactions

UBE2B has been shown to interact with RAD18.^{[3] [4]}

Further reading

• Koken MH, Reynolds P, Jaspers-Dekker I, etal . Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6. . Proc. Natl. Acad. Sci. U.S.A. . 88 . 20 . 8865–9 . 1991 . 1717990 . 10.1073/pnas.88.20.8865 . 52611 . 1991PNAS...88.8865K . free .
• Woffendin C, Chen ZY, Staskus K, etal . Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6. . Biochim. Biophys. Acta . 1090 . 1 . 81–5 . 1991 . 1883845 . 10.1016/0167-4781(91)90039-o.
• Schneider R, Eckerskorn C, Lottspeich F, Schweiger M . The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6. . EMBO J. . 9 . 5 . 1431–5 . 1990 . 2158443 . 10.1002/j.1460-2075.1990.tb08259.x. 551831 .
• Koken MH, Hoogerbrugge JW, Jasper-Dekker I, etal . Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification. . Dev. Biol. . 173 . 1 . 119–32 . 1996 . 8575614 . 10.1006/dbio.1996.0011 . free . 1765/3093 . free .
• Tateishi S, Sakuraba Y, Masuyama S, etal . Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 14 . 7927–32 . 2000 . 10884424 . 10.1073/pnas.97.14.7927 . 16647 . 2000PNAS...97.7927T . free .
• Xin H, Lin W, Sumanasekera W, etal . The human RAD18 gene product interacts with HHR6A and HHR6B. . Nucleic Acids Res. . 28 . 14 . 2847–54 . 2000 . 10908344 . 10.1093/nar/28.14.2847 . 102657 .
• Miura T, Klaus W, Ross A, etal . The NMR structure of the class I human ubiquitin-conjugating enzyme 2b. . J. Biomol. NMR . 22 . 1 . 89–92 . 2003 . 11885984 . 10.1023/A:1013807519703 . 27799378 .
• Shekhar MP, Lyakhovich A, Visscher DW, etal . Rad6 overexpression induces multinucleation, centrosome amplification, abnormal mitosis, aneuploidy, and transformation. . Cancer Res. . 62 . 7 . 2115–24 . 2002 . 11929833 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Kwon YT, Xia Z, An JY, etal . Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway. . Mol. Cell. Biol. . 23 . 22 . 8255–71 . 2003 . 14585983 . 10.1128/MCB.23.22.8255-8271.2003 . 262401 .
• Lyakhovich A, Shekhar MP . RAD6B overexpression confers chemoresistance: RAD6 expression during cell cycle and its redistribution to chromatin during DNA damage-induced response. . Oncogene . 23 . 17 . 3097–106 . 2004 . 14981545 . 10.1038/sj.onc.1207449 . 6180818 .
• Watanabe K, Tateishi S, Kawasuji M, etal . Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination. . EMBO J. . 23 . 19 . 3886–96 . 2005 . 15359278 . 10.1038/sj.emboj.7600383 . 522788 .
• Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
• Stelzl U, Worm U, Lalowski M, etal . A human protein-protein interaction network: a resource for annotating the proteome. . Cell . 122 . 6 . 957–68 . 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . 11858/00-001M-0000-0010-8592-0 . 8235923 . free .
• Shekhar MP, Tait L, Gerard B . Essential role of T-cell factor/beta-catenin in regulation of Rad6B: a potential mechanism for Rad6B overexpression in breast cancer cells. . Mol. Cancer Res. . 4 . 10 . 729–45 . 2007 . 17050667 . 10.1158/1541-7786.MCR-06-0136 . free .

Notes and References

1. Koken MH, Smit EM, Jaspers-Dekker I, Oostra BA, Hagemeijer A, Bootsma D, Hoeijmakers JH . Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31 . Genomics . 12 . 3 . 447–53 . May 1992 . 1559696 . 10.1016/0888-7543(92)90433-S . 1765/3036 . free .
2. Web site: Entrez Gene: UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog).
3. 10.1093/nar/28.14.2847 . Xin . H . Lin W . Sumanasekera W . Zhang Y . Wu X . Wang Z . July 2000 . The human RAD18 gene product interacts with HHR6A and HHR6B . Nucleic Acids Res. . 28 . 14 . 2847–54 . ENGLAND. 10908344 . 102657 .
4. 10.1073/pnas.97.14.7927 . Tateishi . S . Sakuraba Y . Masuyama S . Inoue H . Yamaizumi M . July 2000 . Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens . . 97 . 14 . 7927–32 . UNITED STATES. 0027-8424. 10884424 . 2000PNAS...97.7927T . 16647 . free .