Type I site-specific deoxyribonuclease explained

Type I site-specific deoxyribonuclease
Ec Number:	3.1.21.3
Cas Number:	37263-09-5

Type I site-specific deoxyribonuclease (type I restriction enzyme, deoxyribonuclease (ATP- and S-adenosyl-L-methionine-dependent), restriction-modification system, deoxyribonuclease (adenosine triphosphate-hydrolyzing), adenosine triphosphate-dependent deoxyribonuclease, ATP-dependent DNase, type 1 site-specific deoxyribonuclease) is an enzyme.^[1] This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates; ATP is simultaneously hydrolysed

They have an absolute requirement for ATP (or dATP) and S-adenosyl-L-methionine.

See also

• Restriction enzyme

Notes and References

1. Roberts RJ . Restriction enzymes and their isoschizomers . Nucleic Acids Research . 18 Suppl . 2331–65 . April 1990 . 2159140 . 331877 . 10.1093/nar/18.suppl.2331 .