In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) and distilled water. The solution destroys thered blood cells and platelets within a blood sample (acetic acid being the main lyzing agent), and stains the nuclei of the white blood cells, making them easier to see and count.[1]
Türk's solution is intended for use in determining total leukocyte count in a defined volume of blood. Erythrocytes are hemolyzed while leukocytes are stained for easy visualization.[1] [2]
Composition of Türk's solution is as follows:
| Component | Volume | |
|---|---|---|
| Crystal violet | 1 mL | |
| Glacial acetic acid | 1.5 mL | |
| Distilled water | 100 mL |