| Building Name: | Tsughrughasheni Church წუღრუღაშენის ეკლესია |
| Map Type: | Georgia |
| Map Size: | 275 |
| Location: | Located approximately 2 km from Bolnisi Sioni Basilica, Bolnisi District, Kvemo Kartli Province (Mkhare),  Georgia |
| Coordinates: | 41.3803°N 44.5294°W |
| Religious Affiliation: | Georgian Orthodox Church |
| Region: | Caucasus |
| District: | Bolnisi District |
| Functional Status: | Active |
| Architecture: | yes |
| Architecture Type: | Georgian |
| Founded By: | King George IV Lasha of the Bagrationi dynasty |
| Funded By: | King George IV Lasha of the Bagrationi dynasty |
| Groundbreaking: | 1212 |
| Year Completed: | 1222 |
Tsughrughasheni (Georgian: წუღრუღაშენის ეკლესია) is a Georgian Orthodox church located in the Bolnisi District of Georgia. It is situated approximately 2 kilometres from Bolnisi Sioni basilica, on the right bank of the Bolnisistsqali River. The church was built in 1212–1222 supposedly by King George IV Lasha of the Bagrationi dynasty.
The Tsughrughasheni church resembles stylistically the other Georgian churches from the 12th to 13th century – Betania, Kvatakhevi, Pitareti – but it is smaller than those and has a higher cupola. The plan of the church is right-angled. The church is rich with the Georgian traditional ornaments adorned.