Tre recombinase explained

Tre recombinase is an experimental enzyme that in lab tests has removed DNA inserted by HIV from infected cells.^[1] Through selective mutation, Cre recombinase which recognizes loxP sites are modified to identify HIV long terminal repeats (loxLTR) instead. As a result, instead of performing Cre-Lox recombination, the new enzyme performs recombination at HIV provirus sites.^[2]

The structure of Tre in complex with loxLTR has been resolved, allowing for analyzing the roles of individual mutations.^[3]

External links

• News: Designer enzyme cuts HIV out of infected cells . . June 28, 2007 . JR . Minkel .

Notes and References

1. Indrani . Sarkar . Ilona . Hauber . Joachim . Hauber . Frank . Buchholz . 2007 . HIV-1 proviral DNA excision using an evolved recombinase . . 316 . 5833 . 1912–15 . 17600219 . 10.1126/science.1141453 . 2007Sci...316.1912S . 2437602 .
2. Highly Significant Antiviral Activity of HIV-1 LTR-Specific Tre-Recombinase in Humanized Mice . Ilona . Hauber . Helga . Hofmann-Sieber . Jan . Chemnitz . 3 . Danilo . Dubrau . PLOS Pathogens . 9. 9. e1003587. September 26, 2013 . 10.1371/journal.ppat.1003587 . 24086129. 3784474 . free .
3. Meinke . G . Karpinski . J . Buchholz . F . Bohm . A . Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA. . Nucleic Acids Research . 19 September 2017 . 45 . 16 . 9726–9740 . 10.1093/nar/gkx603 . 28934476 . 5766204 . free.