Torulene dioxygenase explained

Torulene dioxygenase
Ec Number:	1.13.11.59

Torulene dioxygenase (CAO-2, CarT) is an enzyme with systematic name torulene:oxygen oxidoreductase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

torulene + O₂

\rightleftharpoons 4'-apo-beta, psi-caroten-4'-al + 3-methylbut-2-enal

It is assumed that 3-methylbut-2-enal is formed.

Notes and References

1. Prado-Cabrero A, Estrada AF, Al-Babili S, Avalos J . Identification and biochemical characterization of a novel carotenoid oxygenase: elucidation of the cleavage step in the Fusarium carotenoid pathway . Molecular Microbiology . 64 . 2 . 448–60 . April 2007 . 17493127 . 10.1111/j.1365-2958.2007.05665.x . free .
2. Saelices L, Youssar L, Holdermann I, Al-Babili S, Avalos J . Identification of the gene responsible for torulene cleavage in the Neurospora carotenoid pathway . Molecular Genetics and Genomics . 278 . 5 . 527–37 . November 2007 . 17610084 . 10.1007/s00438-007-0269-2 .
3. Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S . Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation . Fungal Genetics and Biology . 45 . 11 . 1497–505 . November 2008 . 18812228 . 10.1016/j.fgb.2008.09.001 .