Tight junction protein ZO-1 explained

Tight junction protein ZO-1 should not be confused with Occludin.

Tight junction protein ZO-1 also known as Zonula occludens-1 (ZO-1), is a 220-kD peripheral membrane protein that is encoded by the TJP1 gene in humans.[1] It belongs to the family of zonula occludens proteins (ZO-1, ZO-2, and ZO-3), which are tight junction-associated proteins and of which, ZO-1 is the first to be cloned. It was first isolated in 1986 by Stevenson and Goodenough using a monoclonal antibody raised in rodent liver to recognise a 225-kD polypeptide in whole liver homogenates and in tight junction-enriched membrane fractions.[2] It has a role as a scaffold protein which cross-links and anchors Tight Junction (TJ) strand proteins, which are fibril-like structures within the lipid bilayer, to the actin cytoskeleton.[3]

Function

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell–cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.[4]

Interactions

Tight junction protein 1 has been shown to interact with:

See also

Further reading

Notes and References

  1. Mohandas TK, Chen XN, Rowe LB, Birkenmeier EH, Fanning AS, Anderson JM, Korenberg JR . Localization of the tight junction protein gene TJP1 to human chromosome 15q13, distal to the Prader-Willi/Angelman region, and to mouse chromosome 7 . Genomics . 30 . 3 . 594–7 . December 1995 . 8825647 . 10.1006/geno.1995.1281 . free .
  2. Stevenson BR, Siliciano JD, Mooseker MS, Goodenough DA . Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia . The Journal of Cell Biology . 103 . 3 . 755–66 . September 1986 . 3528172 . 10.1083/jcb.103.3.755 . 2114282.
  3. Itoh M, Nagafuchi A, Moroi S, Tsukita S . Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments . The Journal of Cell Biology . 138 . 1 . 181–92 . July 1997 . 9214391 . 10.1083/jcb.138.1.181 . 2139940.
  4. Web site: Entrez Gene: TJP1 tight junction protein 1 (zona occludens 1).
  5. Ebnet K, Schulz CU, Meyer Zu Brickwedde MK, Pendl GG, Vestweber D . Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1 . The Journal of Biological Chemistry . 275 . 36 . 27979–88 . Sep 2000 . 10856295 . 10.1074/jbc.M002363200 . free .
  6. Ebnet K, Aurrand-Lions M, Kuhn A, Kiefer F, Butz S, Zander K, Meyer zu Brickwedde MK, Suzuki A, Imhof BA, Vestweber D . The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity . Journal of Cell Science . 116 . Pt 19 . 3879–91 . Oct 2003 . 12953056 . 10.1242/jcs.00704 . free .
  7. Nielsen PA, Baruch A, Shestopalov VI, Giepmans BN, Dunia I, Benedetti EL, Kumar NM . Lens connexins alpha3Cx46 and alpha8Cx50 interact with zonula occludens protein-1 (ZO-1) . Molecular Biology of the Cell . 14 . 6 . 2470–81 . Jun 2003 . 12808044 . 194895 . 10.1091/mbc.E02-10-0637 .
  8. Giepmans BN, Moolenaar WH . The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein . Current Biology . 8 . 16 . 931–4 . 1998 . 9707407 . 10.1016/S0960-9822(07)00375-2 . free .
  9. Liu G, Kaw B, Kurfis J, Rahmanuddin S, Kanwar YS, Chugh SS . Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability . The Journal of Clinical Investigation . 112 . 2 . 209–21 . Jul 2003 . 12865409 . 164293 . 10.1172/JCI18242 .
  10. Huber TB, Schmidts M, Gerke P, Schermer B, Zahn A, Hartleben B, Sellin L, Walz G, Benzing T . The carboxyl terminus of Neph family members binds to the PDZ domain protein zonula occludens-1 . The Journal of Biological Chemistry . 278 . 15 . 13417–21 . Apr 2003 . 12578837 . 10.1074/jbc.C200678200 . free .
  11. Yamamoto T, Harada N, Kawano Y, Taya S, Kaibuchi K . In vivo interaction of AF-6 with activated Ras and ZO-1 . Biochemical and Biophysical Research Communications . 259 . 1 . 103–7 . May 1999 . 10334923 . 10.1006/bbrc.1999.0731 .
  12. Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM . The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton . The Journal of Biological Chemistry . 273 . 45 . 29745–53 . Nov 1998 . 9792688 . 10.1074/jbc.273.45.29745 . free .
  13. Rao RK, Basuroy S, Rao VU, Karnaky Jr KJ, Gupta A . Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by oxidative stress . The Biochemical Journal . 368 . Pt 2 . 471–81 . Dec 2002 . 12169098 . 1222996 . 10.1042/BJ20011804 .
  14. Wittchen ES, Haskins J, Stevenson BR . Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3 . The Journal of Biological Chemistry . 274 . 49 . 35179–85 . Dec 1999 . 10575001 . 10.1074/jbc.274.49.35179 . free .
  15. Itoh M, Morita K, Tsukita S . Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin . The Journal of Biological Chemistry . 274 . 9 . 5981–6 . Feb 1999 . 10026224 . 10.1074/jbc.274.9.5981 . free .

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