Tight junction protein ZO-2 explained

Tight junction protein ZO-2 is a protein that in humans is encoded by the TJP2 gene.^[1]

Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. TJPs bind to the cytoplasmic C termini of junctional transmembrane proteins and link them to the actin cytoskeleton [supplied by OMIM].^[2]

Interactions

Tight junction protein 2 has been shown to interact with tight junction protein 1,^{[3] [4] [5]} band 4.1,^[6] occludin^{[3] [4] [7]} and USP53.^[8]

Further reading

• Denker BM, Nigam SK . Molecular structure and assembly of the tight junction . Am. J. Physiol. . 274 . 1 Pt 2 . F1–9 . 1998 . 9458817 . 10.1152/ajprenal.1998.274.1.F1.
• González-Mariscal L, Betanzos A, Avila-Flores A . MAGUK proteins: structure and role in the tight junction . Semin. Cell Dev. Biol. . 11 . 4 . 315–324 . 2000 . 10966866 . 10.1006/scdb.2000.0178 .
• King JE, Eugenin EA, Buckner CM, Berman JW . HIV tat and neurotoxicity . Microbes Infect. . 8 . 5 . 1347–1357 . 2006 . 16697675 . 10.1016/j.micinf.2005.11.014 . free .
• Van Itallie CM, Balda MS, Anderson JM . Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells . J. Cell Sci. . 108 . 4. 1735–42 . 1995 . 10.1242/jcs.108.4.1735 . 7542259 .
• Beatch M . The tight junction protein ZO-2 contains three PDZ (PSD-95/Discs-Large/ZO-1) domains and an alternatively spliced region . J. Biol. Chem. . 271 . 42 . 25723–25726 . 1996 . 8824195 . 10.1074/jbc.271.42.25723 . vanc. Jesaitis LA . Gallin WJ . 3 . Goodenough . DA . Stevenson . BR . free.
• Itoh M, Morita K, Tsukita S . Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin . J. Biol. Chem. . 274 . 9 . 5981–5986 . 1999 . 10026224 . 10.1074/jbc.274.9.5981 . free .
• Chlenski A . Tight junction protein ZO-2 is differentially expressed in normal pancreatic ducts compared to human pancreatic adenocarcinoma . Int. J. Cancer . 82 . 1 . 137–144 . 1999 . 10360833 . 10.1002/(SICI)1097-0215(19990702)82:1<137::AID-IJC23>3.0.CO;2-F . vanc. Ketels KV . Tsao MS . 3 . Talamonti . Mark S. . Anderson . Marla R. . Oyasu . Ryoichi . Scarpelli . Dante G. . 11349543 .
• Chlenski A . zo-2 gene alternative promoters in normal and neoplastic human pancreatic duct cells . Int. J. Cancer . 83 . 3 . 349–358 . 1999 . 10495427 . 10.1002/(SICI)1097-0215(19991029)83:3<349::AID-IJC10>3.0.CO;2-C . vanc. Ketels KV . Engeriser JL . 3 . Talamonti . Mark S. . Tsao . Ming-Sound . Koutnikova . Hanna . Oyasu . Ryoichi . Scarpelli . Dante G. . free .
• Wittchen ES, Haskins J, Stevenson BR . Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3 . J. Biol. Chem. . 274 . 49 . 35179–35185 . 2000 . 10575001 . 10.1074/jbc.274.49.35179 . free .
• Itoh M . Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins . J. Cell Biol. . 147 . 6 . 1351–1363 . 2000 . 10601346 . 10.1083/jcb.147.6.1351 . 2168087 . vanc. Furuse M . Morita K . 3 . Kubota . K . Saitou . M . Tsukita . S .
• Cordenonsi M . Cingulin contains globular and coiled-coil domains and interacts with ZO-1, ZO-2, ZO-3, and myosin . J. Cell Biol. . 147 . 7 . 1569–1582 . 2000 . 10613913 . 10.1083/jcb.147.7.1569 . 2174252 . vanc. D'Atri F . Hammar E . 3 . Parry . DA . Kendrick-Jones . J . Shore . D . Citi . S .
• Mattagajasingh SN, Huang SC, Hartenstein JS, Benz EJ . Characterization of the interaction between protein 4.1R and ZO-2. A possible link between the tight junction and the actin cytoskeleton . J. Biol. Chem. . 275 . 39 . 30573–30585 . 2000 . 10874042 . 10.1074/jbc.M004578200 . free .
• Chlenski A . Organization and expression of the human zo-2 gene (tjp-2) in normal and neoplastic tissues . Biochim. Biophys. Acta . 1493 . 3 . 319–24 . 2000 . 11018256 . 10.1016/S0167-4781(00)00185-8. vanc. Ketels KV . Korovaitseva GI . 3 . Talamonti . MS . Oyasu . R . Scarpelli . DG .
• Citi S, D'Atri F, Parry DA . Human and Xenopus cingulin share a modular organization of the coiled-coil rod domain: predictions for intra- and intermolecular assembly . J. Struct. Biol. . 131 . 2 . 135–145 . 2000 . 11042084 . 10.1006/jsbi.2000.4284 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–1795 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Glaunsinger BA, Weiss RS, Lee SS, Javier R . Link of the unique oncogenic properties of adenovirus type 9 E4-ORF1 to a select interaction with the candidate tumor suppressor protein ZO-2 . EMBO J. . 20 . 20 . 5578–5586 . 2001 . 11598001 . 10.1093/emboj/20.20.5578 . 125668 .
• Islas S, Vega J, Ponce L, González-Mariscal L . Nuclear localization of the tight junction protein ZO-2 in epithelial cells . Exp. Cell Res. . 274 . 1 . 138–148 . 2002 . 11855865 . 10.1006/excr.2001.5457 .

Notes and References

1. Duclos F, Rodius F, Wrogemann K, Mandel JL, Koenig M . The Friedreich ataxia region: characterization of two novel genes and reduction of the critical region to 300 kb . Hum Mol Genet . 3 . 6 . 909–914 . November 1994 . 7951235 . 10.1093/hmg/3.6.909 .
2. Web site: Entrez Gene: TJP2 tight junction protein 2 (zona occludens 2).
3. Itoh . M . Morita K . Tsukita S . February 1999 . Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin . J. Biol. Chem. . 274 . 9 . 5981–5986 . 0021-9258. 10026224 . 10.1074/jbc.274.9.5981 . free .
4. Wittchen . E S . Haskins J . Stevenson B R . December 1999 . Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3 . J. Biol. Chem. . 274 . 49 . 35179–35185 . 0021-9258. 10575001 . 10.1074/jbc.274.49.35179 . free .
5. Fanning . A S . Jameson B J . Jesaitis L A . Anderson J M . November 1998 . The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton . J. Biol. Chem. . 273 . 45 . 29745–29753 . 0021-9258. 9792688 . 10.1074/jbc.273.45.29745 . free .
6. Mattagajasingh . S N . Huang S C . Hartenstein J S . Benz E J . September 2000 . Characterization of the interaction between protein 4.1R and ZO-2. A possible link between the tight junction and the actin cytoskeleton . J. Biol. Chem. . 275 . 39 . 30573–30585 . 0021-9258. 10874042 . 10.1074/jbc.M004578200 . free .
7. Peng . Bi-Hung . Lee J Ching . Campbell Gerald A . December 2003 . In vitro protein complex formation with cytoskeleton-anchoring domain of occludin identified by limited proteolysis . J. Biol. Chem. . 278 . 49 . 49644–49651 . 0021-9258. 14512431 . 10.1074/jbc.M302782200 . free .
8. Kazmierczak M, Harris SL, Kazmierczak P, Shah P, Starovoytov V, Ohlemiller KK, Schwander M . Progressive Hearing Loss in Mice Carrying a Mutation in Usp53 . The Journal of Neuroscience . 35 . 47 . 15582–98 . November 2015 . 26609154 . 4659823 . 10.1523/JNEUROSCI.1965-15.2015 .