| Thomas Wylie | |
| Office1: | Ontario MPP |
| Term Start1: | 1886 |
| Term End1: | 1894 |
| Predecessor1: | Orson James Phelps |
| Successor1: | Archibald Currie |
| Constituency1: | Simcoe West |
| Party: | Conservative |
| Birth Date: | 4 June 1841 |
| Birth Place: | Victoria, Chinguacousy Township, Peel County, Canada West |
| Death Place: | York, Ontario, Canada |
| Occupation: | Physician |
Thomas Wylie (June 4, 1841 - July 10, 1915) was an Ontario physician and political figure. He represented Simcoe West in the Legislative Assembly of Ontario as a Conservative member from 1886 to 1894.
He was born in Toronto in 1841, the son of John Wylie, a Scottish immigrant. Wylie studied medicine at John Rolph's Toronto School of Medicine, graduating with an M.D. in 1866. In 1867, he married Catherine Ferguson. He practiced medicine in Toronto and Stayner.