| Thomas Twining Keefler | |
| Constituency Mp: | Lunenburg |
| Parliament: | Canadian |
| Predecessor: | Charles Edwin Kaulbach |
| Successor: | Charles Edwin Kaulbach |
| Term Start: | 1882 |
| Term End: | 1883 |
| Birth Date: | 26 March 1824 |
| Birth Place: | Halifax, Nova Scotia |
| Party: | Liberal |
Thomas Twining Keefler (March 26, 1824 - November 16, 1906) was a merchant and political figure in Nova Scotia, Canada. He represented Lunenburg in the House of Commons of Canada from 1882 to 1883 as a Liberal member.
He was born in Halifax, of Austrian descent. In 1870, he married Lydia Sophia Tupper. Keefler supported an elected senate. His election in 1882 was declared void and he was defeated by Charles Edwin Kaulbach in the by-election which followed in 1883.