Thiocyanate hydrolase explained
| thiocyanate hydrolase |
| Ec Number: | 3.5.5.8 |
| Go Code: | 0018760 |
A thiocyanate hydrolase is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase.This enzyme catalyzes the chemical reaction:
The mechanism is proposed to involve a metal thiocyanate complex.
Structural studies
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes,,, and .
A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:[1]
References
- Katayama Y, Matsushita Y, Kaneko M, Kondo M, Mizuno T, Nyunoya H . 1998 . Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase . J. Bacteriol. . 180 . 2583 - 9 . 9573140 . 10 . 10.1128/JB.180.10.2583-2589.1998 . 107207 .
- Katayama Y, Narahara Y, Inoue Y, Amano F, Kanagawa T, Kuraishi H . 1992 . A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate . J. Biol. Chem. . 267 . 9170 - 5 . 1577754 . 13 .
Notes and References
- 10.1073/pnas.1922133117 . Trinuclear Copper Biocatalytic Center Forms an Active Site of Thiocyanate Dehydrogenase . 2020 . Tikhonova . Tamara V. . Sorokin . Dimitry Y. . Hagen . Wilfred R. . Khrenova . Maria G. . Muyzer . Gerard . Rakitina . Tatiana V. . Shabalin . Ivan G. . Trofimov . Anton A. . Tsallagov . Stanislav I. . Popov . Vladimir O. . Proceedings of the National Academy of Sciences . 117 . 10 . 5280–5290 . free . 32094184 . 2020PNAS..117.5280T .
