Thiamine oxidase explained

thiamin oxidase
Ec Number:	1.1.3.23
Cas Number:	96779-44-1
Go Code:	0050423

In enzymology, a thiamine oxidase is an enzyme that catalyzes the chemical reaction

thiamine + 2 O₂ + H₂O

\rightleftharpoons thiamine acetic acid + 2 H₂O₂

The 3 substrates of this enzyme are thiamine, O₂, and H₂O, whereas its two products are thiamine acetic acid and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is thiamine:oxygen 5-oxidoreductase. Other names in common use include thiamin dehydrogenase, thiamine dehydrogenase, and thiamin:oxygen 5-oxidoreductase. This enzyme participates in thiamine metabolism. It employs one cofactor, FAD.

References

• Edmondson DE, Kenney WC, Singer TP . 1976 . Structural elucidation and properties of 8alpha-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and beta-cyclopiazonate oxidocyclase . Biochemistry . 15 . 2937 - 45 . 8076 . 10.1021/bi00659a001 . 14 .
• Gomez-Moreno C, Edmondson DE . 1985 . Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase . Arch. Biochem. Biophys. . 239 . 46 - 52 . 2988447 . 10.1016/0003-9861(85)90810-0 . 1 .
• Neal RA . 1970 . Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2'-methyl-4'-amino-5'-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism . J. Biol. Chem. . 245 . 2599 - 604 . 4987737 . 10 .