| Theodore Otis | |
| Order: | 7th |
| Office: | Mayor of Roxbury, Massachusetts |
| Term Start: | 1859 |
| Term End: | 1860 |
| Predecessor: | John Sherburne Sleeper |
| Successor: | William Gaston |
| Order2: | Massachusetts House of Representatives for the 4th Norfolk District, |
| Birth Date: | December 15, 1810 |
| Death Date: | July 11, 1873 |
| Nationality: | American |
| Party: | Free Soil |
| Spouse: | Harriet Blanchard |
| Children: | James Otis |
| Alma Mater: | Union College |
| Profession: | Attorney |
Theodore Otis (December 15, 1810 – July 11, 1873) was an American politician, who served as a member of the Massachusetts House of Representatives and the seventh Mayor of Roxbury, Massachusetts from 1859 to 1860.