| Jurisdiction: | Territorial Prelature |
| Santiago Apóstol de Huancané | |
| Latin: | Praelatura Territorialis Iacobus Apostolus Huancanensis |
| Country: |  Peru |
| Rite: | Latin Rite |
| Cathedral: | Santiago Apóstol Cathedral in Huancané |
| Area Km2: | 18,881 |
| Population: | 200,489 |
| Population As Of: | 2019 |
| Catholics: | 170,415 |
| Catholics Percent: | 85.0 |
| Bishop: | Giovanni Cefai, M.S.S.P. |
The Territorial Prelature of Santiago Apóstol de Huancané (Latin: Praelatura Territorialis Iacobus Apostolus Huancanensis) is a Roman Catholic territorial prelature located in the city of Huancané in the ecclesiastical province of Arequipa in Peru.
On 3 April 2019, the Territorial Prelature of Santiago Apóstol de Huancané was established from the Roman Catholic Territorial Prelature of Ayaviri and Roman Catholic Territorial Prelature of Juli.