Taxadiene 5alpha-hydroxylase explained

taxadiene 5-alpha-hydroxylase
Ec Number:	1.14.99.37
Cas Number:	9035-51-2
Go Code:	0050604

In enzymology, a taxadiene 5alpha-hydroxylase is an enzyme that catalyzes the chemical reaction

taxa-4,11-diene + AH₂ + O₂

\rightleftharpoons taxa-4(20),11-dien-5alpha-ol + A + H₂O

The 3 substrates of this enzyme are taxa-4,11-diene, an electron acceptor AH₂, and O₂, whereas its 3 products are taxa-4(20),11-dien-5alpha-ol, the reduction product A, and H₂O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derive from O miscellaneous. The systematic name of this enzyme class is taxa-4,11-diene,hydrogen-donor:oxygen oxidoreductase (5alpha-hydroxylating). This enzyme participates in diterpenoid biosynthesis.

Further reading

• Hefner J, Rubenstein SM, Ketchum RE, Gibson DM, Williams RM, Croteau R . Cytochrome P450-catalyzed hydroxylation of taxa-4(5),11(12)-diene to taxa-4(20),11(12)-dien-5alpha-ol: the first oxygenation step in taxol biosynthesis . Chemistry & Biology . 3 . 6 . 479–89 . June 1996 . 8807878 . 10.1016/S1074-5521(96)90096-4 . free .