TST (gene) explained

Thiosulfate sulfurtransferase is an enzyme that in humans is encoded by the TST gene.^{[1] [2]}

The product of this gene is a mitochondrial matrix enzyme that is encoded by the nucleus. It may play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes.

The gene product contains two highly conservative domains (rhodanese homology domains), suggesting these domains have a common evolutionary origin.

Further reading

• Pecci L, Pensa B, Costa M, etal . Reaction of rhodanese with dithiothreitol . Biochim. Biophys. Acta . 445 . 1 . 104–11 . 1976 . 986188 . 10.1016/0005-2744(76)90163-7.
• Polo CF, Vazquez ES, Caballero F, etal . Heme biosynthesis pathway regulation in a model of hepatocarcinogenesis pre-initiation . Comp. Biochem. Physiol. B . 103 . 1 . 251–6 . 1993 . 1451437 . 10.1016/0305-0491(92)90440-3 .
• Lewis JL, Rhoades CE, Gervasi PG, etal . The cyanide-metabolizing enzyme rhodanese in human nasal respiratory mucosa . Toxicol. Appl. Pharmacol. . 108 . 1 . 114–20 . 1991 . 2006499 . 10.1016/0041-008X(91)90274-I .
• Malliopoulou VA, Rakitzis ET, Malliopoulou TB . Inactivation of rhodanese from human gastric mucosa and stomach adenocarcinoma by 2,4, 6-trinitrobenzenesulphonate and by 4,4'-diisothiocyanatostilbene-2,2'-disulphonate . Anticancer Res. . 9 . 4 . 1133–6 . 1989 . 2817794 .
• Vazquez E, Buzaleh AM, Wider E, Batlle AM . Red blood cell rhodanese: its possible role in modulating delta-aminolaevulinate synthetase activity in mammals . Int. J. Biochem. . 19 . 2 . 217–9 . 1987 . 3471602 . 10.1016/0020-711X(87)90337-5 .
• Pallini R, Martelli P, Bardelli AM, etal . Normal rhodanese activity in leukocytes from Leber patients: enzyme characterization and activity levels . Neurology . 37 . 12 . 1878–80 . 1987 . 3479705 . 10.1212/wnl.37.12.1878. 35839633 .
• Pagani S, Galante YM . Interaction of rhodanese with mitochondrial NADH dehydrogenase . Biochim. Biophys. Acta . 742 . 2 . 278–84 . 1983 . 6402020 . 10.1016/0167-4838(83)90312-6.
• Mimori Y, Nakamura S, Kameyama M . Regional and subcellular distribution of cyanide metabolizing enzymes in the central nervous system . J. Neurochem. . 43 . 2 . 540–5 . 1984 . 6588145 . 10.1111/j.1471-4159.1984.tb00932.x . 10055954 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Merrill GA, Butler M, Horowitz PM . Limited tryptic digestion near the amino terminus of bovine liver rhodanese produces active electrophoretic variants with altered refolding . J. Biol. Chem. . 268 . 21 . 15611–20 . 1993 . 10.1016/S0021-9258(18)82300-6 . 8340386 . free .
• Aita N, Ishii K, Akamatsu Y, etal . Cloning and expression of human liver rhodanese cDNA . Biochem. Biophys. Res. Commun. . 231 . 1 . 56–60 . 1997 . 9070219 . 10.1006/bbrc.1996.6046 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Dunham I, Shimizu N, Roe BA, etal . The DNA sequence of human chromosome 22 . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
• Wieprecht T, Apostolov O, Beyermann M, Seelig J . Interaction of a mitochondrial presequence with lipid membranes: role of helix formation for membrane binding and perturbation . Biochemistry . 39 . 50 . 15297–305 . 2001 . 11112515 . 10.1021/bi001774v .
• Picton R, Eggo MC, Merrill GA, etal . Mucosal protection against sulphide: importance of the enzyme rhodanese . Gut . 50 . 2 . 201–5 . 2002 . 11788560 . 10.1136/gut.50.2.201 . 1773108 .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Gevaert K, Goethals M, Martens L, etal . Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides . Nat. Biotechnol. . 21 . 5 . 566–9 . 2004 . 12665801 . 10.1038/nbt810 . 23783563 .
• Kwiecień I, Sokołowska M, Luchter-Wasylewska E, Włodek L . Inhibition of the catalytic activity of rhodanese by S-nitrosylation using nitric oxide donors . Int. J. Biochem. Cell Biol. . 35 . 12 . 1645–57 . 2004 . 12962704 . 10.1016/S1357-2725(03)00005-0 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .

Notes and References

1. Pallini R, Guazzi GC, Cannella C, Cacace MG . Cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes . Biochem Biophys Res Commun . 180 . 2 . 887–93 . Dec 1991 . 1953758 . 10.1016/S0006-291X(05)81148-9 . free .
2. Web site: Entrez Gene: TST thiosulfate sulfurtransferase (rhodanese).