TRNT1 explained

tRNA-nucleotidyltransferase 1, is an enzyme that in humans is encoded by the TRNT1 gene.^{[1] [2] [3]} This enzyme adds the nucleotide sequence CCA to the 3' end of tRNA, using ATP and CTP as substrates. The sequence creates the binding site for an amino acid.^[4]

Further reading

• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Wiemann S . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 . vanc. Weil B . Wellenreuther R . 3 . Gassenhuber . J . Glassl . S . Ansorge . W . Böcher . M . Blöcker . H . Bauersachs . S .
• Simpson JC . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Rep. . 1 . 3 . 287–92 . 2001 . 11256614 . 10.1093/embo-reports/kvd058 . 1083732 . vanc. Wellenreuther R . Poustka A . 3 . Pepperkok . R . Wiemann . S .
• Reichert AS, Thurlow DL, Mörl M . A eubacterial origin for the human tRNA nucleotidyltransferase? . Biol. Chem. . 382 . 10 . 1431–8 . 2002 . 11727826 . 10.1515/BC.2001.176 . 10.1.1.657.1235 . 230616 .
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . vanc. Feingold EA . Grouse LH . 3 . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .
• Augustin MA . Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization . J. Mol. Biol. . 328 . 5 . 985–94 . 2003 . 12729736 . 10.1016/S0022-2836(03)00381-4 . vanc. Reichert AS . Betat H . 3 . Huber . Robert . Mörl . Mario . Steegborn . Clemens . free .
• Gerhard DS . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . vanc. Wagner L . Feingold EA . 3 . Shenmen . CM . Grouse . LH . Schuler . G . Klein . SL . Old . S . Rasooly . R .
• Wiemann S . From ORFeome to biology: a functional genomics pipeline . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . vanc. Arlt D . Huber W . 3 . Wellenreuther . R . Schleeger . S . Mehrle . A . Bechtel . S . Sauermann . M . Korf . U .
• Kimura K . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 . vanc. Wakamatsu A . Suzuki Y . 3 . Ota . T . Nishikawa . T . Yamashita . R . Yamamoto . J . Sekine . M . Tsuritani . K .
• Mehrle A . The LIFEdb database in 2006 . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 . vanc. Rosenfelder H . Schupp I . 3 . Del Val . C . Arlt . D . Hahne . F . Bechtel . S . Simpson . J . Hofmann . O .
• Lizano E . A splice variant of the human CCA-adding enzyme with modified activity . J. Mol. Biol. . 366 . 4 . 1258–65 . 2007 . 17204286 . 10.1016/j.jmb.2006.12.016 . vanc. Schuster J . Müller M . 3 . Kelso . J . Morl . M .

Notes and References

1. Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W . Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics . Genome Res . 10 . 5 . 703–13 . Aug 2000 . 10810093 . 310876 . 10.1101/gr.10.5.703 .
2. Nagaike T, Suzuki T, Tomari Y, Takemoto-Hori C, Negayama F, Watanabe K, Ueda T . Identification and characterization of mammalian mitochondrial tRNA nucleotidyltransferases . J Biol Chem . 276 . 43 . 40041–9 . Oct 2001 . 11504732 . 10.1074/jbc.M106202200 . free .
3. Web site: Entrez Gene: TRNT1 tRNA nucleotidyl transferase, CCA-adding, 1.
4. Lizano E, Scheibe M, Rammelt C, Betat H, Mörl M . A comparative analysis of CCA-adding enzymes from human and E. coli: differences in CCA addition and tRNA 3'-end repair . Biochimie . 90 . 5 . 762–72 . May 2008 . 18226598 . 10.1016/j.biochi.2007.12.007.