TRNA pseudouridine32 synthase explained

tRNA pseudouridine^32 synthase
Ec Number:	5.4.99.28
Cas Number:	430429-15-5

tRNA pseudouridine³² synthase (RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine³² uracil mutase.^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction

tRNA uridine³²

\rightleftharpoons tRNA pseudouridine³²

The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine⁷⁴⁶ in 23S rRNA.

Notes and References

1. Hoang C, Chen J, Vizthum CA, Kandel JM, Hamilton CS, Mueller EG, Ferré-D'Amaré AR . Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure . Molecular Cell . 24 . 4 . 535–45 . November 2006 . 17188032 . 10.1016/j.molcel.2006.09.017 . free .
2. Spedaliere CJ, Hamilton CS, Mueller EG . Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues . Biochemistry . 39 . 31 . 9459–65 . August 2000 . 10924141 . 10.1021/bi001079n .
3. Raychaudhuri S, Niu L, Conrad J, Lane BG, Ofengand J . Functional effect of deletion and mutation of the Escherichia coli ribosomal RNA and tRNA pseudouridine synthase RluA . The Journal of Biological Chemistry . 274 . 27 . 18880–6 . July 1999 . 10383384 . 10.1074/jbc.274.27.18880 . free .
4. Ramamurthy V, Swann SL, Spedaliere CJ, Mueller EG . Role of cysteine residues in pseudouridine synthases of different families . Biochemistry . 38 . 40 . 13106–11 . October 1999 . 10529181 . 10.1021/bi9913911 .
5. Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J . A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe) . RNA . 1 . 4 . 437–48 . June 1995 . 7493321 . 1482406 .
6. Behm-Ansmant I, Grosjean H, Massenet S, Motorin Y, Branlant C . Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae . The Journal of Biological Chemistry . 279 . 51 . 52998–3006 . December 2004 . 15466869 . 10.1074/jbc.m409581200 . 13197875 . free .