TRNA pseudouridine13 synthase explained

tRNA pseudouridine^13 synthase
Ec Number:	5.4.99.27
Cas Number:	430429-15-5

tRNA pseudouridine¹³ synthase (TruD, YgbO, tRNA PSI13 synthase, RNA:PSI-synthase Pus7p, Pus7p, RNA:pseudouridine-synthase Pus7p, Pus7 protein) is an enzyme with systematic name tRNA-uridine¹³ uracil mutase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

tRNA uridine¹³

\rightleftharpoons tRNA pseudouridine¹³

Pseudouridine synthase TruD from Escherichia coli specifically acts on uridine¹³ in tRNA.

Notes and References

1. Ericsson UB, Nordlund P, Hallberg BM . X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold . FEBS Letters . 565 . 1–3 . 59–64 . May 2004 . 15135053 . 10.1016/j.febslet.2004.03.085 . 31602560 .
2. Chan CM, Huang RH . Enzymatic characterization and mutational studies of TruD--the fifth family of pseudouridine synthases . Archives of Biochemistry and Biophysics . 489 . 1–2 . 15–9 . September 2009 . 19664587 . 10.1016/j.abb.2009.07.023 .
3. Kaya Y, Ofengand J . A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya . RNA . 9 . 6 . 711–21 . June 2003 . 12756329 . 1370438 . 10.1261/rna.5230603 .
4. Behm-Ansmant I, Urban A, Ma X, Yu YT, Motorin Y, Branlant C . The Saccharomyces cerevisiae U2 snRNA:pseudouridine-synthase Pus7p is a novel multisite-multisubstrate RNA:Psi-synthase also acting on tRNAs . RNA . 9 . 11 . 1371–82 . November 2003 . 14561887 . 1287059 . 10.1261/rna.5520403 .
5. Urban A, Behm-Ansmant I, Branlant C, Motorin Y . RNA sequence and two-dimensional structure features required for efficient substrate modification by the Saccharomyces cerevisiae RNA:-synthase Pus7p . The Journal of Biological Chemistry . 284 . 9 . 5845–58 . February 2009 . 19114708 . 10.1074/jbc.m807986200 . 19586610 . free .