TRNA (uracil-5-)-methyltransferase explained

tRNA (uracil-5-)-methyltransferase
Ec Number:	2.1.1.35
Cas Number:	37257-02-6
Go Code:	0009021

In enzymology, a tRNA (uracil-5-)-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA containing uridine at position 54

\rightleftharpoons S-adenosyl-L-homocysteine + tRNA containing ribothymidine at position 54

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA containing uridine at position 54, whereas its two products are S-adenosylhomocysteine and tRNA containing ribothymidine at position 54.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (uracil-5-)-methyltransferase. Other names in common use include ribothymidyl synthase, transfer RNA uracil 5-methyltransferase, transfer RNA uracil methylase, tRNA uracil 5-methyltransferase, m5U-methyltransferase, tRNA:m5U54-methyltransferase, and RUMT.

References

• Hurwitz J, Gold M, Anders M . 1964 . The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes . J. Biol. Chem. . 239 . 3462 - 3473 . 14245404 .
• Bjork GR, Svensson I . 1969 . Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae . Eur. J. Biochem. . 9 . 207 - 15 . 4896260 . 10.1111/j.1432-1033.1969.tb00596.x . 2 . free .
• Greenberg R, Dudock B . 1980 . Isolation and characterization of m5U-methyltransferase from Escherichia coli . J. Biol. Chem. . 255 . 8296 - 302 . 6997293 . 17 .
• Delk AS, Nagle DP, Rabinowitz JC . 1980 . Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2 . J. Biol. Chem. . 255 . 4387 - 90 . 6768721 . 10 .
• Kealey JT, Gu X, Santi DV . 1994 . Enzymatic mechanism of tRNA (m5U54)methyltransferase . Biochimie . 76 . 1133 - 42 . 7748948 . 10.1016/0300-9084(94)90042-6 . 12 .
• Gu X, Ivanetich KM, Santi DV . 1996 . Recognition of the T-arm of tRNA by tRNA (m5U54)-methyltransferase is not sequence specific . Biochemistry . 35 . 11652 - 9 . 8794745 . 10.1021/bi9612125 . 36 .
• Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H . 1997 . Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs . J. Mol. Biol. . 274 . 505 - 18 . 9417931 . 10.1006/jmbi.1997.1417 . 4 .