TRNA (guanine-N7-)-methyltransferase explained

tRNA (guanine-N7-)-methyltransferase
Ec Number:	2.1.1.33
Cas Number:	37257-00-4
Go Code:	0008176

In enzymology, a tRNA (guanine-N7-)-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + tRNA containing N₇-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N7-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, and S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .

References

• Hurwitz J, Gold M, Anders M . 1964 . The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes . J. Biol. Chem. . 239 . 3462 - 3473 . 14245404 .
• Bjork GR, Svensson I . 1969 . Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae . Eur. J. Biochem. . 9 . 207 - 215 . 4896260 . 10.1111/j.1432-1033.1969.tb00596.x . 2 . free .
• Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H . 1976 . 7-Methylguanine specific tRNA-methyltransferase from Escherichia coli . Nucleic Acids Res. . 3 . 3109 - 22 . 794833 . 11 . 343155 . 10.1093/nar/3.11.3109.
• Book: Salvatore F, Traboni C, Colonna A, Ciliberto G, Paoletta C, Cimino F . 1980 . Natural Sulfur Compounds . Cavallini D, Gaul GE, Zappia V . Plenum Press . New York . 25 .