TRNA (guanine-N2-)-methyltransferase explained

tRNA (guanine-N2-)-methyltransferase
Ec Number:	2.1.1.32
Cas Number:	37217-31-5
Go Code:	0004809

In enzymology, a tRNA (guanine-N2-)-methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + tRNA containing N₂-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N2-Methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N2-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 2-methyltransferase, transfer ribonucleate guanine N2-methyltransferase, transfer RNA guanine 2-methyltransferase, guanine-N2-methylase, and S-adenosyl-L-methionine:tRNA (guanine-2-N-)-methyltransferase.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes,, and .

References

• Baguley BC, Staehelin M . 1968 . The specificity of transfer ribonucleic acid methylases from rat liver . Biochemistry . 7 . 45 - 50 . 4921283 . 10.1021/bi00841a007 . 1 .
• Bjork GR, Svensson I . 1969 . Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae . Eur. J. Biochem. . 9 . 207 - 15 . 4896260 . 10.1111/j.1432-1033.1969.tb00596.x . 2 . free .
• Krauss J, Stahelin M. 1974 . N2-Guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen* . Nucleic Acids Res. . 1 . 1455 - 1478 . 10.1093/nar/1.11.1455 . 10793703 . 11 . 343424.
• Krauss J, Stahelin M. 1974 . N2-Guanine specific transfer RNA methyltransferase II from rat liver . Nucleic Acids Res. . 1 . 1479 - 1496 . 10.1093/nar/1.11.1479 . 10793704 . 11 . 343425.
• Agris PF, Spremulli LL, Brown GM . 1974 . tRNA methylases from HeLa cells: purification and properties of an adenine-1-methylase and a guanine-N2-methylase . Arch. Biochem. Biophys. . 162 . 38 - 47 . 4208689 . 10.1016/0003-9861(74)90102-7 . 1 .
• Glick JM, Ross S, Leboy PS . 1975 . S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver . Nucleic Acids Res. . 2 . 1639 - 51 . 1208211 . 10.1093/nar/2.10.1639 . 10 . 343534 .
• Izzo P, Gantt R . 1977 . Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos . Biochemistry . 16 . 3576 - 81 . 19056 . 10.1021/bi00635a012 . 16 .
• Glick JM, Averyhart VM, Leboy PS . 1978 . Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver . Biochim. Biophys. Acta . 518 . 158 - 71 . 629973 . 1 . 10.1016/0005-2787(78)90125-9.