TRNA (guanine-N1-)-methyltransferase explained
| tRNA (guanine-N1-)-methyltransferase |
| Ec Number: | 2.1.1.31 |
| Cas Number: | 37256-99-8 |
| Go Code: | 0009019 |
In enzymology, a tRNA (guanine-N1-)-methyltransferase is an enzyme that catalyzes the chemical reaction
S-adenosyl-L-methionine + tRNA
S-adenosyl-L-homocysteine + tRNA containing N
1-methylguanine
Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N1-methylguanine.
This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N1-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 1-methyltransferase, tRNA guanine 1-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanine-1-N-)-methyltransferase.
Structural studies
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes,,,,, and .
References
- Hurwitz J, Gold M, Anders M . 1964 . The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes . J. Biol. Chem. . 239 . 3462 - 3473 . 10.1016/S0021-9258(18)97746-X . free . 14245404 .
- Bjork GR, Svensson I . 1969 . Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae . Eur. J. Biochem. . 9 . 207 - 215 . 4896260 . 10.1111/j.1432-1033.1969.tb00596.x . 2 . free .
- Smolar N, Hellman U, Svensson I . 1975 . Two transfer RNA (1-methylguanine) methylases from yeast . Nucleic Acids Res. . 2 . 993 - 1004 . 1096087 . 10.1093/nar/2.6.993 . 6 . 343485 .
- Glick JM, Averyhart VM, Leboy PS . 1978 . Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver . Biochim. Biophys. Acta . 518 . 158 - 171 . 629973 . 1 . 10.1016/0005-2787(78)90125-9.
