TRNA (cytidine34-2'-O)-methyltransferase explained

tRNA (cytidine^34-2'-O)-methyltransferase
Ec Number:	2.1.1.207

tRNA (cytidine³⁴-2'-O)-methyltransferase (yibK (gene), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytidine^34/5-carboxymethylaminomethyluridine³⁴-2'-O)-methyltransferase.^[1] This enzyme catalyses the following chemical reaction

\rightleftharpoons S-adenosyl-L-homocysteine + 2'-O-methylcytidine³⁴ in tRNA

(2) S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine³⁴ in tRNALeu

\rightleftharpoons S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine³⁴ in tRNALeu

The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA.

Notes and References

1. Benítez-Páez A, Villarroya M, Douthwaite S, Gabaldón T, Armengod ME . YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors . RNA . 16 . 11 . 2131–43 . November 2010 . 20855540 . 2957053 . 10.1261/rna.2245910 .