TRNA (adenine57-N1/adenine58-N1)-methyltransferase explained

TRNA (adenine^57-N^1/adenine^58-N^1)-methyltransferase
Ec Number:	2.1.1.219

TRNA (adenine⁵⁷-N¹/adenine⁵⁸-N¹)-methyltransferase (TrmI, PabTrmI, AqTrmI, MtTrmI) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (adenine⁵⁷/adenine⁵⁸-N¹)-methyltransferase.^{[1] [2]} This enzyme catalyses the following chemical reaction:

2 S-adenosyl-L-methionine + adenine⁵⁷/adenine⁵⁸ in tRNA

\rightleftharpoons 2 S-adenosyl-L-homocysteine + N¹-methyladenine⁵⁷/N¹-methyladenine⁵⁸ in tRNA

The enzyme catalyses the formation of N¹-methyladenine at two adjacent positions (57 and 58) in the T-loop of certain tRNAs .

Notes and References

1. Roovers M, Wouters J, Bujnicki JM, Tricot C, Stalon V, Grosjean H, Droogmans L . A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase . Nucleic Acids Research . 32 . 2 . 465–76 . 2004 . 14739239 . 373318 . 10.1093/nar/gkh191 .
2. Guelorget A, Roovers M, Guérineau V, Barbey C, Li X, Golinelli-Pimpaneau B . Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m1A57/58 methyltransferase . Nucleic Acids Research . 38 . 18 . 6206–18 . October 2010 . 20483913 . 2952851 . 10.1093/nar/gkq381 .