TRNA-pseudouridine synthase I explained

tRNA-pseudouridine synthase
Ec Number:	5.4.99.12
Go Code:	0016439

In enzymology, a tRNA-pseudouridine synthase I is an enzyme that catalyzes the chemical reaction

tRNA uridine

\rightleftharpoons tRNA pseudouridine

Hence, this enzyme has one substrate, tRNA uridine, and one product, tRNA pseudouridine.

This enzyme belongs to the family of isomerases, specifically those intramolecular transferases transferring other groups. The systematic name of this enzyme class is tRNA-uridine uracilmutase. Other names in common use include tRNA-uridine isomerase, tRNA pseudouridylate synthase I, transfer ribonucleate pseudouridine synthetase, pseudouridine synthase, and transfer RNA pseudouridine synthetase.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes,,, and .

References

• Arena F, Ciliberto G, Ciampi S, Cortese R . 1978 . Purification of pseudouridylate synthetase I from Salmonella typhimurium . Nucleic Acids Res. . 5 . 4523 - 36 . 370771 . 10.1093/nar/5.12.4523 . 12 . 342770 .
• Kammen HO, Marvel CC, Hardy L, Penhoet EE . 1988 . Purification, structure, and properties of Escherichia coli tRNA pseudouridine synthase I . J. Biol. Chem. . 263 . 2255 - 63 . 3276686 . 5 . 10.1016/S0021-9258(18)69199-9 . free .