TRIOBP explained
TRIO and F-actin-binding protein is a protein that in humans is encoded by the TRIOBP gene.[1] [2] [3] [4]
This gene encodes a protein that interacts with Trio, which is involved with neural tissue development and in controlling actin cytoskeleton organization, cell motility, and cell growth. This trio-binding protein also associates with F-actin and stabilizes F-actin structures. Domains contained in this encoded protein are an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. Mutations in this gene have been associated with a form of autosomal-recessive nonsyndromic deafness. Multiple alternatively-spliced transcript variants that would encode different isoforms have been found for this gene, though some transcripts may be subject to nonsense-mediated decay (NMD).[4]
Further reading
- Nakajima D, Okazaki N, Yamakawa H, etal . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. . DNA Res. . 9 . 3 . 99–106 . 2003 . 12168954 . 10.1093/dnares/9.3.99 . free .
- Mayer BJ, Ren R, Clark KL, Baltimore D . A putative modular domain present in diverse signaling proteins. . Cell . 73 . 4 . 629–30 . 1993 . 8500161 . 10.1016/0092-8674(93)90244-K . 44282241 .
- Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
- Ueki N, Oda T, Kondo M, etal . Selection system for genes encoding nuclear-targeted proteins. . Nat. Biotechnol. . 16 . 13 . 1338–42 . 1999 . 9853615 . 10.1038/4315 . 20001769 .
- Dunham I, Shimizu N, Roe BA, etal . The DNA sequence of human chromosome 22. . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
- Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination. . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
- Hirosawa M, Nagase T, Murahashi Y, etal . Identification of novel transcribed sequences on human chromosome 22 by expressed sequence tag mapping. . DNA Res. . 8 . 1 . 1–9 . 2001 . 11258795 . 10.1093/dnares/8.1.1 . free .
- Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
- Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
- Gerhard DS, Wagner L, Feingold EA, etal . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 .
- Wiemann S, Arlt D, Huber W, etal . From ORFeome to biology: a functional genomics pipeline. . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 .
- Mehrle A, Rosenfelder H, Schupp I, etal . The LIFEdb database in 2006. . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 .
- Olsen JV, Blagoev B, Gnad F, etal . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . free .
- Li X, Lan J, Zhu Y, etal . Expression, purification, and characterization of Tara, a novel telomere repeat-binding factor 1 (TRF1)-binding protein. . Protein Expr. Purif. . 55 . 1 . 84–92 . 2007 . 17629495 . 10.1016/j.pep.2007.05.004 .
Notes and References
- Seipel K, O'Brien SP, Iannotti E, Medley QG, Streuli M . Tara, a novel F-actin binding protein, associates with the Trio guanine nucleotide exchange factor and regulates actin cytoskeletal organization . J Cell Sci . 114 . Pt 2 . 389–99 . Jan 2001 . 10.1242/jcs.114.2.389 . 11148140 .
- Riazuddin S, Khan SN, Ahmed ZM, Ghosh M, Caution K, Nazli S, Kabra M, Zafar AU, Chen K, Naz S, Antonellis A, Pavan WJ, Green ED, Wilcox ER, Friedman PL, Morell RJ, Riazuddin S, Friedman TB . Mutations in TRIOBP, which encodes a putative cytoskeletal-organizing protein, are associated with nonsyndromic recessive deafness . Am J Hum Genet . 78 . 1 . 137–43 . Dec 2005 . 16385457 . 1380211 . 10.1086/499164 .
- Shahin H, Walsh T, Sobe T, Abu Sa'ed J, Abu Rayan A, Lynch ED, Lee MK, Avraham KB, King MC, Kanaan M . Mutations in a novel isoform of TRIOBP that encodes a filamentous-actin binding protein are responsible for DFNB28 recessive nonsyndromic hearing loss . Am J Hum Genet . 78 . 1 . 144–52 . Dec 2005 . 16385458 . 1380212 . 10.1086/499495 .
- Web site: Entrez Gene: TRIOBP TRIO and F-actin binding protein.