Perilipin-3 Explained

Mannose-6-phosphate receptor binding protein 1 (M6PRBP1) is a protein which in humans is encoded by the M6PRBP1 gene.^[1] Its gene product, as well as the gene itself, is commonly known as TIP47.

Function

Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo.^[2]

The mannose-6-phosphate receptor-binding properties of TIP47 are disputed,^[3] despite the designation of M6PRBP1 as TIP47's gene symbol. TIP47 protein is most commonly described in the scientific literature as a coat protein for lipid droplets.^[4]

TIP47 belongs to the peripilin protein family and shares significant homology with the other genes of this family, including perilipin and adipophilin.^[5]

Interactions

M6PRBP1 has been shown to interact with both Mannose 6-phosphate receptors.^{[6] [7]}

Further reading

• Bohn H, Kraus W, Winckler W . Purification and characterization of two new soluble placental tissue proteins (PP13 and PP17). . Oncodev. Biol. Med. . 4 . 5 . 343–50 . 1983 . 6856484 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, etal . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Díaz E, Pfeffer SR . TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking. . Cell . 93 . 3 . 433–43 . 1998 . 9590177 . 10.1016/S0092-8674(00)81171-X . 17161071 . free .
• Than NG, Sumegi B, Than GN, etal . Cloning and sequence analysis of cDNAs encoding human placental tissue protein 17 (PP17) variants. . Eur. J. Biochem. . 258 . 2 . 752–7 . 1999 . 9874244 . 10.1046/j.1432-1327.1998.2580752.x . free .
• Than NG, Sumegi B, Than GN, etal . Cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17): homology with adipophilin and the mouse adipose differentiation-related protein. . Tumour Biol. . 20 . 4 . 184–92 . 1999 . 10393528 . 10.1159/000030062 . 46847947 .
• Krise JP, Sincock PM, Orsel JG, Pfeffer SR . Quantitative analysis of TIP47-receptor cytoplasmic domain interactions: implications for endosome-to-trans Golgi network trafficking. . J. Biol. Chem. . 275 . 33 . 25188–93 . 2000 . 10829017 . 10.1074/jbc.M001138200 . free .
• Orsel JG, Sincock PM, Krise JP, Pfeffer SR . Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 16 . 9047–51 . 2000 . 10908666 . 10.1073/pnas.160251397 . 16819 . 2000PNAS...97.9047O . free .
• Barbero P, Buell E, Zulley S, Pfeffer SR . TIP47 is not a component of lipid droplets. . J. Biol. Chem. . 276 . 26 . 24348–51 . 2001 . 11313361 . 10.1074/jbc.M102468200 . free .
• Carroll KS, Hanna J, Simon I, etal . Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. . Science . 292 . 5520 . 1373–6 . 2001 . 11359012 . 10.1126/science.1056791 . 2001Sci...292.1373C . 31343936 .
• Hanna J, Carroll K, Pfeffer SR . Identification of residues in TIP47 essential for Rab9 binding. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 11 . 7450–4 . 2002 . 12032303 . 10.1073/pnas.112198799 . 124251 . 2002PNAS...99.7450H . free .
• Miura S, Gan JW, Brzostowski J, etal . Functional conservation for lipid storage droplet association among Perilipin, ADRP, and TIP47 (PAT)-related proteins in mammals, Drosophila, and Dictyostelium. . J. Biol. Chem. . 277 . 35 . 32253–7 . 2002 . 12077142 . 10.1074/jbc.M204410200 . free .
• Strausberg RL, Feingold EA, Grouse LH, etal . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . free .
• Sincock PM, Ganley IG, Krise JP, etal . Self-assembly is important for TIP47 function in mannose 6-phosphate receptor transport. . Traffic . 4 . 1 . 18–25 . 2003 . 12535272 . 10.1034/j.1600-0854.2003.40104.x . 27015300 .
• Than NG, Sumegi B, Bellyei S, etal . Lipid droplet and milk lipid globule membrane associated placental protein 17b (PP17b) is involved in apoptotic and differentiation processes of human epithelial cervical carcinoma cells. . Eur. J. Biochem. . 270 . 6 . 1176–88 . 2003 . 12631276 . 10.1046/j.1432-1033.2003.03475.x . free .
• Nair P, Schaub BE, Rohrer J . Characterization of the endosomal sorting signal of the cation-dependent mannose 6-phosphate receptor. . J. Biol. Chem. . 278 . 27 . 24753–8 . 2003 . 12697764 . 10.1074/jbc.M300174200 . free .
• Blot G, Janvier K, Le Panse S, etal . Targeting of the human immunodeficiency virus type 1 envelope to the trans-Golgi network through binding to TIP47 is required for env incorporation into virions and infectivity. . J. Virol. . 77 . 12 . 6931–45 . 2003 . 12768012 . 10.1128/JVI.77.12.6931-6945.2003 . 156179 .
• Ota T, Suzuki Y, Nishikawa T, etal . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . free .
• Colland F, Jacq X, Trouplin V, etal . Functional proteomics mapping of a human signaling pathway. . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 10.1101/gr.2334104 . 442148 .
• Ganley IG, Carroll K, Bittova L, Pfeffer S . Rab9 GTPase regulates late endosome size and requires effector interaction for its stability. . Mol. Biol. Cell . 15 . 12 . 5420–30 . 2005 . 15456905 . 10.1091/mbc.E04-08-0747 . 532021 .

Notes and References

1. Web site: Entrez Gene: M6PRBP1 mannose-6-phosphate receptor binding protein 1.
2. Carroll KS, Hanna J, Simon I, Krise J, Barbero P, Pfeffer SR . Role of Rab9 GTPase in facilitating receptor recruitment by TIP47. . Science . 292. 5520 . 1373–6 . May 2001. 11359012 . 10.1126/science.1056791. 2001Sci...292.1373C. 31343936 .
3. Bulankina AV, Deggerich A, Wenzel D, Mutenda K, Wittmann JG, Rudolph MG, Burger KN, Höning S . TIP47 functions in the biogenesis of lipid droplets . J. Cell Biol. . 185 . 4 . 641–55 . May 2009 . 19451273 . 10.1083/jcb.200812042 . 2711566 .
4. Ducharme NA, Bickel PE . Lipid droplets in lipogenesis and lipolysis . Endocrinology . 149 . 3 . 942–9 . March 2008 . 18202123 . 10.1210/en.2007-1713 . free .
5. Brasaemle DL . Thematic review series: adipocyte biology. The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis . J. Lipid Res. . 48 . 12 . 2547–59 . December 2007 . 17878492 . 10.1194/jlr.R700014-JLR200 . 38744670 . free .
6. 10.1016/S0092-8674(00)81171-X . Díaz . E . Pfeffer S R . May 1998 . TIP47: a cargo selection device for mannose 6-phosphate receptor trafficking . Cell . 93 . 3 . 433–43 . UNITED STATES. 0092-8674. 9590177 . 17161071 . free .
7. Orsel . J G . Sincock P M . Krise J P . Pfeffer S R . August 2000 . Recognition of the 300-kDa mannose 6-phosphate receptor cytoplasmic domain by 47-kDa tail-interacting protein . . 97 . 16 . 9047–51 . UNITED STATES. 0027-8424. 10908666 . 10.1073/pnas.160251397 . 2000PNAS...97.9047O . 16819 . free .