TBCD explained

Tubulin-specific chaperone D is a protein that in humans is encoded by the TBCD gene.^[1]

Function

Cofactor D is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state.

Interactions

TBCD has been shown to interact with ARL2.^{[2] [3]}

Further reading

• Lewis SA, Tian G, Vainberg IE, Cowan NJ . Chaperonin-mediated folding of actin and tubulin . J. Cell Biol. . 132 . 1–2 . 1–4 . 1996 . 8567715 . 2120700 . 10.1083/jcb.132.1.1 .
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Tian G, Huang Y, Rommelaere H, Vandekerckhove J, Ampe C, Cowan NJ . Pathway leading to correctly folded beta-tubulin . Cell . 86 . 2 . 287–96 . 1996 . 8706133 . 10.1016/S0092-8674(00)80100-2 . 18359371 . free .
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA . Large-scale concatenation cDNA sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 139146 . 10.1101/gr.7.4.353 .
• Tian G, Lewis SA, Feierbach B, Stearns T, Rommelaere H, Ampe C, Cowan NJ . Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors . J. Cell Biol. . 138 . 4 . 821–32 . 1997 . 9265649 . 2138046 . 10.1083/jcb.138.4.821 .
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro . DNA Res. . 6 . 1 . 63–70 . 1999 . 10231032 . 10.1093/dnares/6.1.63 . free .
• Martín L, Fanarraga ML, Aloria K, Zabala JC . Tubulin folding cofactor D is a microtubule destabilizing protein . FEBS Lett. . 470 . 1 . 93–5 . 2000 . 10722852 . 10.1016/S0014-5793(00)01293-X . 35012741 . free .
• Bhamidipati A, Lewis SA, Cowan NJ . ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin . J. Cell Biol. . 149 . 5 . 1087–96 . 2000 . 10831612 . 2174823 . 10.1083/jcb.149.5.1087 .
• Schubert A, Cattaruzza M, Hecker M, Darmer D, Holtz J, Morawietz H . Shear stress-dependent regulation of the human beta-tubulin folding cofactor D gene . Circ. Res. . 87 . 12 . 1188–94 . 2001 . 11110777 . 10.1161/01.res.87.12.1188 . free .
• Wistow G, Bernstein SL, Wyatt MK, Fariss RN, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K . Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants . Mol. Vis. . 8 . 205–20 . 2002 . 12107410 .
• Shern JF, Sharer JD, Pallas DC, Bartolini F, Cowan NJ, Reed MS, Pohl J, Kahn RA . Cytosolic Arl2 is complexed with cofactor D and protein phosphatase 2A . J. Biol. Chem. . 278 . 42 . 40829–36 . 2003 . 12912990 . 10.1074/jbc.M308678200 . free .
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM . Functional proteomics mapping of a human signaling pathway . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 442148 . 10.1101/gr.2334104 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Web site: Entrez Gene: TBCD tubulin folding cofactor D.
2. Shern JF, Sharer JD, Pallas DC, Bartolini F, Cowan NJ, Reed MS, Pohl J, Kahn RA . Cytosolic Arl2 is complexed with cofactor D and protein phosphatase 2A . J. Biol. Chem. . 278 . 42 . 40829–36 . October 2003 . 12912990 . 10.1074/jbc.M308678200 . free .
3. Bhamidipati A, Lewis SA, Cowan NJ . ADP ribosylation factor-like protein 2 (Arl2) regulates the interaction of tubulin-folding cofactor D with native tubulin . J. Cell Biol. . 149 . 5 . 1087–96 . May 2000 . 10831612 . 2174823 . 10.1083/jcb.149.5.1087 .