TAF1A explained

TATA box-binding protein-associated factor RNA polymerase I subunit A is an enzyme that in humans is encoded by the TAF1A gene.^{[1] [2]}

Function

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as selective factor 1 (SL1), binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Interactions

TAF1A has been shown to interact with Acidic leucine-rich nuclear phosphoprotein 32 family member A^[3] and Protein SET.^[3]

Further reading

• Rudloff U, Eberhard D, Tora L, Stunnenberg H, Grummt I . TBP-associated factors interact with DNA and govern species specificity of RNA polymerase I transcription . EMBO J. . 13 . 11 . 2611–6 . 1994 . 10.1002/j.1460-2075.1994.tb06551.x . 8013460 . 395135 .
• Di Pietro C, Rapisarda A, Amico V, Bonaiuto C, Viola A, Scalia M, Motta S, Amato A, Engel H, Messina A, Sichel G, Grzeschik K, Purrello M . Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1 . Cytogenet. Cell Genet. . 89 . 1–2 . 133–6 . 2000 . 10894955 . 10.1159/000015592 . 27014629 .
• Zhai W, Comai L . Repression of RNA polymerase I transcription by the tumor suppressor p53 . Mol. Cell. Biol. . 20 . 16 . 5930–8 . 2000 . 10913176 . 86070 . 10.1128/MCB.20.16.5930-5938.2000 .
• Seo SB, McNamara P, Heo S, Turner A, Lane WS, Chakravarti D . Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein . Cell . 104 . 1 . 119–30 . 2001 . 11163245 . 10.1016/S0092-8674(01)00196-9 . 11144887 . free .
• Miller G, Panov KI, Friedrich JK, Trinkle-Mulcahy L, Lamond AI, Zomerdijk JC . hRRN3 is essential in the SL1-mediated recruitment of RNA Polymerase I to rRNA gene promoters . EMBO J. . 20 . 6 . 1373–82 . 2001 . 11250903 . 145519 . 10.1093/emboj/20.6.1373 .
• Dynes JL, Xu S, Bothner S, Lahti JM, Hori RT . The carboxyl-terminus directs TAF(I)48 to the nucleus and nucleolus and associates with multiple nuclear import receptors . J. Biochem. . 135 . 3 . 429–38 . 2004 . 15113842 . 10.1093/jb/mvh051 .
• Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M . Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription . Mol. Cell. Biol. . 24 . 14 . 6338–49 . 2004 . 15226435 . 434256 . 10.1128/MCB.24.14.6338-6349.2004 .
• Grandori C, Gomez-Roman N, Felton-Edkins ZA, Ngouenet C, Galloway DA, Eisenman RN, White RJ . c-Myc binds to human ribosomal DNA and stimulates transcription of rRNA genes by RNA polymerase I . Nat. Cell Biol. . 7 . 3 . 311–8 . 2005 . 15723054 . 10.1038/ncb1224 . 8913931 .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .

Notes and References

1. Comai L, Zomerdijk JC, Beckmann H, Zhou S, Admon A, Tjian R . Reconstitution of transcription factor SL1: exclusive binding of TBP by SL1 or TFIID subunits . Science . 266 . 5193 . 1966–72 . December 1994 . 7801123 . 10.1126/science.7801123 . 1994Sci...266.1966C .
2. Web site: Entrez Gene: TAF1A TATA box binding protein (TBP)-associated factor, RNA polymerase I, A, 48kDa.
3. Seo SB, McNamara P, Heo S, Turner A, Lane WS, Chakravarti D . Regulation of histone acetylation and transcription by INHAT, a human cellular complex containing the set oncoprotein . Cell . 104 . 1 . 119–30 . January 2001 . 11163245 . 10.1016/S0092-8674(01)00196-9 . 11144887 . free .