TADA3L explained

Transcriptional adapter 3-like is a protein that in humans is encoded by the TADA3 gene.^{[1] [2] [3]} Cytogenetic location: 3p25.3 ^[4]

Function

Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined.

Interactions

TADA3L has been shown to interact with:

• Retinoic acid receptor alpha,^[5]
• Retinoid X receptor alpha,^[5]
• TADA2L,
• TAF9,^[6]
• Transcription initiation protein SPT3 homolog,^[6] and
• USP5.^[7]

Further reading

• Struhl K, Moqtaderi Z . The TAFs in the HAT . Cell . 94 . 1 . 1–4 . Jul 1998 . 9674419 . 10.1016/S0092-8674(00)81213-1 . 14869072 . free .
• Brand M, Yamamoto K, Staub A, Tora L . Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction . The Journal of Biological Chemistry . 274 . 26 . 18285–9 . Jun 1999 . 10373431 . 10.1074/jbc.274.26.18285 . free .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Research . 10 . 11 . 1788–95 . Nov 2000 . 11076863 . 310948 . 10.1101/gr.143000 .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Research . 11 . 3 . 422–35 . Mar 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Reports . 1 . 3 . 287–92 . Sep 2000 . 11256614 . 1083732 . 10.1093/embo-reports/kvd058 .
• Brand M, Moggs JG, Oulad-Abdelghani M, Lejeune F, Dilworth FJ, Stevenin J, Almouzni G, Tora L . UV-damaged DNA-binding protein in the TFTC complex links DNA damage recognition to nucleosome acetylation . The EMBO Journal . 20 . 12 . 3187–96 . Jun 2001 . 11406595 . 150203 . 10.1093/emboj/20.12.3187 .
• Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG . Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo . Molecular and Cellular Biology . 21 . 20 . 6782–95 . Oct 2001 . 11564863 . 99856 . 10.1128/MCB.21.20.6782-6795.2001 .
• Benecke A, Gaudon C, Garnier JM, vom Baur E, Chambon P, Losson R . ADA3-containing complexes associate with estrogen receptor alpha . Nucleic Acids Research . 30 . 11 . 2508–14 . Jun 2002 . 12034840 . 117179 . 10.1093/nar/30.11.2508 .
• Kumar A, Zhao Y, Meng G, Zeng M, Srinivasan S, Delmolino LM, Gao Q, Dimri G, Weber GF, Wazer DE, Band H, Band V . Human papillomavirus oncoprotein E6 inactivates the transcriptional coactivator human ADA3 . Molecular and Cellular Biology . 22 . 16 . 5801–12 . Aug 2002 . 12138191 . 133989 . 10.1128/MCB.22.16.5801-5812.2002 .
• Zeng M, Kumar A, Meng G, Gao Q, Dimri G, Wazer D, Band H, Band V . Human papilloma virus 16 E6 oncoprotein inhibits retinoic X receptor-mediated transactivation by targeting human ADA3 coactivator . The Journal of Biological Chemistry . 277 . 47 . 45611–8 . Nov 2002 . 12235159 . 10.1074/jbc.M208447200 . free .
• Bernhard Landwehrmeyer . Goehler H, Lalowski M, Stelzl U, Waelter S, Stroedicke M, Worm U, Droege A, Lindenberg KS, Knoblich M, Haenig C, Herbst M, Suopanki J, Scherzinger E, Abraham C, Bauer B, Hasenbank R, Fritzsche A, Ludewig AH, Büssow K, Buessow K, Coleman SH, Gutekunst CA, Landwehrmeyer BG, Lehrach H, Wanker EE . A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease . Molecular Cell . 15 . 6 . 853–65 . Sep 2004 . 15383276 . 10.1016/j.molcel.2004.09.016 . free .
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A . From ORFeome to biology: a functional genomics pipeline . Genome Research . 14 . 10B . 2136–44 . Oct 2004 . 15489336 . 528930 . 10.1101/gr.2576704 .
• Meng G, Zhao Y, Nag A, Zeng M, Dimri G, Gao Q, Wazer DE, Kumar R, Band H, Band V . Human ADA3 binds to estrogen receptor (ER) and functions as a coactivator for ER-mediated transactivation . The Journal of Biological Chemistry . 279 . 52 . 54230–40 . Dec 2004 . 15496419 . 10.1074/jbc.M404482200 . free .

Notes and References

1. Ogryzko VV, Kotani T, Zhang X, Schiltz RL, Howard T, Yang XJ, Howard BH, Qin J, Nakatani Y . Histone-like TAFs within the PCAF histone acetylase complex . Cell . 94 . 1 . 35–44 . Jul 1998 . 9674425 . 10.1016/S0092-8674(00)81219-2 . 18942972 . free .
2. Wang T, Kobayashi T, Takimoto R, Denes AE, Snyder EL, el-Deiry WS, Brachmann RK . hADA3 is required for p53 activity . The EMBO Journal . 20 . 22 . 6404–13 . Nov 2001 . 11707411 . 125723 . 10.1093/emboj/20.22.6404 .
3. Web site: Entrez Gene: TADA3L transcriptional adaptor 3 (NGG1 homolog, yeast)-like.
4. Sulimova G, Kutsenko A, Rakhmanaliev E, Udina I, Kompaniytsev A, Protopopov A, Moisjak E, Klimov E, Muravenko O, Zelenin A, Braga E, Kashuba V, Zabarovsky E, Kisselev L . Human chromosome 3: integration of 60 NotI clones into a physical and gene map . Cytogenetic and Genome Research. 98 . 2–3. 177–183 . 2002. 12698000. 10.1159/000069814 . 41010053 .
5. Zeng M, Kumar A, Meng G, Gao Q, Dimri G, Wazer D, Band H, Band V . Human papilloma virus 16 E6 oncoprotein inhibits retinoic X receptor-mediated transactivation by targeting human ADA3 coactivator . The Journal of Biological Chemistry . 277 . 47 . 45611–8 . Nov 2002 . 12235159 . 10.1074/jbc.M208447200 . free .
6. Martinez E, Palhan VB, Tjernberg A, Lymar ES, Gamper AM, Kundu TK, Chait BT, Roeder RG . Human STAGA complex is a chromatin-acetylating transcription coactivator that interacts with pre-mRNA splicing and DNA damage-binding factors in vivo . Molecular and Cellular Biology . 21 . 20 . 6782–95 . Oct 2001 . 11564863 . 99856 . 10.1128/MCB.21.20.6782-6795.2001 .
7. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .