PACSIN2 explained

Protein kinase C and casein kinase substrate in neurons protein 2 (Pacsin 2) is a protein that in humans is encoded by the PACSIN2 gene.^{[1] [2] [3]} Pacsin 2 is involved in the formation of caveolae.^[4]

The variant rs2413739 in PACSIN2 has been associated with the activity of the enzyme thiopurine-methyltransferase during remission maintenance therapy of children with acute lymphoblastic leukemia and with the incidence of severe gastrointestinal adverse events during remission consolidation therapy of children with acute lymphoblastic leukemia, based mostly on the antimetabolites mercaptopurine and methotrexate.^[5]

Interactions

PACSIN2 has been shown to interact with:

• Fas ligand,^[6]
• PACSIN1,^[7] and
• PACSIN3.^[2]

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 .
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA . Large-scale concatenation cDNA sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 139146 . 10.1101/gr.7.4.353 .
• Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP . The DNA sequence of human chromosome 22 . Nature . 402 . 6761 . 489–95 . 1999 . 10591208 . 10.1038/990031 . 1999Natur.402..489D . free .
• Sumoy L, Pluvinet R, Andreu N, Estivill X, Escarceller M . PACSIN 3 is a novel SH3 domain cytoplasmic adapter protein of the pacsin-syndapin-FAP52 gene family . Gene . 262 . 1–2 . 199–205 . 2001 . 11179684 . 10.1016/S0378-1119(00)00531-X .
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 311072 . 10.1101/gr.GR1547R .
• Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O . Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178) . FEBS Lett. . 519 . 1–3 . 50–8 . 2002 . 12023017 . 10.1016/S0014-5793(02)02709-6 . 26765451 .
• Collins JE, Wright CL, Edwards CA, Davis MP, Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y, Huckle EJ, Beare DM, Dunham I . A genome annotation-driven approach to cloning the human ORFeome . Genome Biol. . 5 . 10 . R84 . 2005 . 15461802 . 545604 . 10.1186/gb-2004-5-10-r84 . free .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Kessels MM, Dong J, Leibig W, Westermann P, Qualmann B . Complexes of syndapin II with dynamin II promote vesicle formation at the trans-Golgi network . J. Cell Sci. . 119 . Pt 8 . 1504–16 . 2006 . 16551695 . 10.1242/jcs.02877 . free .
• Cuajungco MP, Grimm C, Oshima K, D'hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S . PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4 . J. Biol. Chem. . 281 . 27 . 18753–62 . 2006 . 16627472 . 10.1074/jbc.M602452200 . free . 2066/51163 . free .
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . free .

Notes and References

1. Ritter B, Modregger J, Paulsson M, Plomann M . PACSIN 2, a novel member of the PACSIN family of cytoplasmic adapter proteins . FEBS Lett . 454 . 3 . 356–62 . Aug 1999 . 10431838 . 10.1016/S0014-5793(99)00830-3 . 44788141 . free .
2. Modregger J, Ritter B, Witter B, Paulsson M, Plomann M . All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis . J Cell Sci . 113 . 24. 4511–21 . Jan 2001 . 10.1242/jcs.113.24.4511 . 11082044 .
3. Web site: Entrez Gene: PACSIN2 protein kinase C and casein kinase substrate in neurons 2.
4. Shvets. E. Ludwig. A. Nichols. BJ. News from the caves: update on the structure and function of caveolae.. Current Opinion in Cell Biology. August 2014. 29. 99–106. 24908346. 10.1016/j.ceb.2014.04.011.
5. PACSIN2 rs2413739 Influence on Thiopurine Pharmacokinetics: Validation Studies in Pediatric Patients . The Pharmacogenomics Journal . 2020 . 10.1038/s41397-019-0130-0. 2318/1757668 . free . Franca . Raffaella . Stocco . Gabriele . Favretto . Diego . Giurici . Nagua . Del Rizzo . Irene . Locatelli . Franco . Vinti . Luciana . Biondi . Andrea . Colombini . Antonella . Fagioli . Franca . Barisone . Elena . Pelin . Marco . Martellossi . Stefano . Ventura . Alessandro . Decorti . Giuliana . Rabusin . Marco . 20 . 3 . 415–425 . 31792371 . 208539277 .
6. Ghadimi MP, Sanzenbacher R, Thiede B, Wenzel J, Jing Q, Plomann M, Borkhardt A, Kabelitz D, Janssen O . Identification of interaction partners of the cytosolic polyproline region of CD95 ligand (CD178) . FEBS Lett. . 519 . 1–3 . 50–8 . May 2002 . 12023017 . 10.1016/s0014-5793(02)02709-6. 26765451 .
7. Huda Zoghbi . Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . Oct 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .