| Event: | Women's 100 metre backstroke S14 |
| Games: | 2016 Summer |
| Venue: | Olympic Aquatics Stadium |
| Dates: | 8 September |
| Competitors: | 9 |
| Nations: | 8 |
| Gold: | Bethany Firth |
| Goldnpc: | GBR |
| Silver: | Marlou van der Kulk |
| Silvernpc: | NED |
| Bronze: | Jessica-Jane Applegate |
| Bronzenpc: | GBR |
The women's 100 metre backstroke S14 event at the 2016 Paralympic Games took place on 8 September, at the Olympic Aquatics Stadium. Two heats were held, the first with four swimmer and the second with five swimmers. The swimmers with the eight fastest times advanced to the final.
, the existing World and Paralympic records were as follows.
| World record | 1:04.70 | Glasgow, Great Britain | 28 March 2015 | ||
| Paralympic record | 1:08.93 | London, Great Britain | 31 August 2012 | ||
| 2016 World leading | 1:04.73 | Sheffield, Great Britain | 27 July 2016 |
| Rank | Lane | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 1 | 4 | 1:07.23 | Q | |||
| 2 | 3 | 1:15.54 | Q | |||
| 3 | 5 | 1:16.26 | Q | |||
| 4 | 6 | 1:19.05 |
| Rank | Lane | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 1 | 4 | 1:04.53 | Q, WR | |||
| 2 | 5 | 1:08.41 | Q | |||
| 3 | 6 | 1:14.14 | Q | |||
| 4 | 3 | 1:16.80 | Q | |||
| 5 | 2 | 1:18.26 | Q |
| Rank | Lane | Name | Nationality | Time | Notes | |
|---|---|---|---|---|---|---|
| 4 | 1:04.05 | WR | ||||
| 5 | 1:06.33 | |||||
| 3 | 1:08.67 | |||||
| 4 | 6 | 1:13.95 | ||||
| 5 | 2 | 1:15.40 | ||||
| 6 | 7 | 1:16.02 | ||||
| 7 | 1 | 1:16.76 | ||||
| 8 | 8 | 1:17.64 |